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. 2022 Aug 19:5:1287-1294.
doi: 10.1016/j.crfs.2022.08.004. eCollection 2022.

Phenotypic and genotypic characterisation of Lactobacillus and yeast isolates from a traditional New Zealand Māori potato starter culture

Jia Sun  1 , Olin Silander  2 , Kay Rutherfurd-Markwick  3 , Daying Wen  2 , Tanya Poi-Poi Davy  4 , Anthony N Mutukumira  1
Affiliations

Phenotypic and genotypic characterisation of Lactobacillus and yeast isolates from a traditional New Zealand Māori potato starter culture

Jia Sun et al. Curr Res Food Sci. .

Abstract

Parāroa Rēwena is a traditional Māori sourdough produced by fermentation using a potato starter culture. The microbial composition of the starter culture is not well characterised, despite the long history of this product. The morphological, physiological, biochemical and genetic tests were conducted to characterise 26 lactic acid bacteria (LAB) and 15 yeast isolates from a Parāroa Rēwena potato starter culture. The results of sugar fermentation tests, API 50 CHL tests, and API ID 32 C tests suggest the presence of four different LAB phenotypes and five different yeast phenotypes. 16S rRNA and 26S rRNA sequencing identified the LAB as Lacticaseibacillus paracasei and the yeast isolates as Saccharomyces cerevisiae, respectively. Multilocus sequence typing (MLST) of the L. paracasei isolates indicated that they had identical genotypes at the MLST loci, to L. paracasei subsp. paracasei IBB 3423 or L. paracasei subsp. paracasei F19. This study provides new insights into the microbial composition of the traditional sourdough Parāroa Rēwena starter culture.

Keywords: Lacticaseibacillus paracasei; Lactobacillus; MLST; Oxford Nanopore sequencing; Rēwena parāoa sourdough bread; Saccharomyces cerevisiae.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Image 1
Graphical abstract
Fig. 1
Fig. 1
Maximum likelihood tree based on the concatenated MLST sequences of Lactobacillus paracasei isolates. Note: The Rēwena isolate is highlighted in bold. The scale bar shows the number of substitutions per base pair across a 4262 base pair alignment. The tree is midpoint rooted, and bootstrap values are shown at each node.
See this image and copyright information in PMC

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