A successful case of assisted reproduction after contamination of the culture medium with Escherichia coli

Abstract

Introduction: We report a case of successful pregnancy after in vitro fertilization culture (IVF) medium contaminated with Escherichia coli (E. coli).

Case report: To remove E. coli, oocytes were isolated in a PVP-ICSI medium and then in a culture medium supplemented with gentamicin. We selected two embryos that were vitrified and frozen. In the next natural cycle, we performed the embryo transfer of 2 embryos.

Results: Only one pregnancy was uncomplicated until term and was completed by the live birth of a healthy baby. The child's subsequent development until the date of publication of this article is normal.

Conclusions: In cases of E. coli contamination of the IVF culture medium, specific laboratory methods allow the procedure to be continued, and a healthy baby can be obtained.