Analysis for Sister Chromatid Exchange
- PMID: 36066711
- DOI: 10.1007/978-1-0716-2433-3_8
Analysis for Sister Chromatid Exchange
Abstract
Sister chromatid exchange (SCE) is the exchange event of genetic material between two identical sister chromatid. Elevation of SCE frequency is considered as a result of replication stress from genetic defects, ROS stress, and genomic damages. SCE staining needs extra processes compared to regular Giemsa staining. Usually two rounds of cell cycle progress are required to observe SCE under microscope. SCE can be visualized with the fluorescence plus Giemsa (FPG) staining method or fluorescence staining methods with immunocytochemistry to BrdU or Click reaction to EdU which provide more clear images of SCE. This chapter will provide the detailed method for the SCE staining and measurement for the traditional FPG staining, BrdU monoclonal antibody staining method, and newly developed EdU Click reaction staining method.
Keywords: BrdU; EdU; Replication stress; Sister chromatid exchange.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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References
-
- Taylor JH, Woods PS, Hughes WL (1957) The organization and duplication of chromosomes as revealed by autoradiographic studies using tritium-labeled Thymidinee. Proc Natl Acad Sci U S A 43(1):122–128 - DOI
-
- Perry P, Wolff S (1974) New Giemsa method for the differential staining of sister chromatids. Nature 251(5471):156–158 - DOI
-
- Latt SA (1973) Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes. Proc Natl Acad Sci U S A 70(12):3395–3399 - DOI
-
- Raza A, Kempski M, Preisler HD, Block AW (1985) A permanent method of detecting Sce by immunofluorescence using monoclonal anti-Brdu antibodies. Cancer Genet Cytogen 15(1–2):187–189
-
- Sunada S, Haskins JS, Kato TA (2019) Sister chromatid exchange as a genotoxic stress marker. Methods Mol Biol 1984:61–68. https://doi.org/10.1007/978-1-4939-9432-8_7 - DOI - PubMed
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