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. 2022 Nov;19(11):1314-1316.
doi: 10.1038/s41423-022-00920-y. Epub 2022 Sep 7.

Cbf-β is required for the development, differentiation, and function of murine mucosal-associated invariant T cells

Tingting Liu #  1   2   3 , Jugmohit S Toor #  1   2   4 , Kalpana Subedi  1   2 , Jie Wang  1   2 , Qijun Yi  1   2 , Ian Loveless  1   2 , Li Zhou  5   6   7 , Qing-Sheng Mi  8   9   10
Affiliations

Cbf-β is required for the development, differentiation, and function of murine mucosal-associated invariant T cells

Tingting Liu et al. Cell Mol Immunol. 2022 Nov.
No abstract available

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Cbf-β is required for murine MAIT cell development, differentiation, and function. a Analysis of Cbf-β expression in scRNA-seq data from Koay et al. [7]. b The MAIT cell frequencies in different organs of CD4cre Cbf-βfl/fl (Cbf-β KO) mice were compared to those in littermate control mice through analysis of the indicated tetramers. Cells were gated on live single cells. c The percentage (top) and absolute number (bottom) of MAIT cells from b. d Enriched thymic MAIT cells were gated and stained with the indicated antibodies to determine developmental stages in Cbf-β KO mice and littermate control mice. e MAIT cells from different organs of Cbf-β KO mice and littermate control mice were stained with T-bet and RORγt to identify and quantify MAIT1 and MAIT17 cells, respectively. Stimulated hepatic MAIT cells isolated from UBC Cre-ERT2 Cbf-βfl/fl mice after tamoxifen induction were stained with f an anti-CD69 antibody to measure activation and g an anti-GZMB antibody to measure cytotoxicity. UBC + Cbf-β indicates mice with inducible Cbf-β knockdown, and UBC-Cbf-β indicates mice without inducible knockdown. The data are representative of at least 2 independent experiments. Each dot represents one mouse. At least three mice were used for each group. The data were analyzed by an unpaired t test. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.00001 compared to WT
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References

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