Effect of Leukoreduced Platelet Rich Plasma on Intra-Articular Pro-Inflammatory Cytokines in a Canine Pilot Study

Abstract

Evaluation of effect of Leukoreduced Platelet Rich Plasma (LrPRP) on TNF-α and IL-6 (pro-inflammatory cytokines) in joint fluid in dogs with cranial cruciate ligament rupture (CCLR). Eight client-owned dogs with CCLR were assigned to treatment (2 mL LrPRP) or control (2 mL saline) injection groups. Day of evaluation (day 0) and day of surgery (day 10-14), joint fluid was collected and joint injected. Joint fluid was also collected on day of suture removal (day 20-28). TNF-α and IL-6 concentrations of joint fluid were measured using a bead-based antibody assay. Concentrations at the later time points were expressed as a ratio to the initial level within each stifle. LrPRP had a mean concentration of platelets 1.7 times higher that of whole blood on day of evaluation and 1.4 times higher on day of the surgery. Leukocytes were reduced by 99.7%. On day of surgery, TNF-α ratios in the joint fluid from dogs injected with LrPRP were significantly different that TNF-α ratios of control group. On the day of suture removal ratios of IL-6 and TNF-α were lower in LrPRP treatment group compared with control group; however, differences were not significant. LrPRP modulate ratios of pro-inflammatory cytokine TNF-α in dogs with CCLR.

Keywords: cytokines; dog; osteoarthritis; platelet rich plasma; stifle.

Figure 1

Evolution of TNF-a between intra-articular injection of LrPRP group and the saline group at the time of day of surgery and day of suture removal when compared with day of evaluation. * Significant difference between groups p = 0.0294.

Figure 2

Evolution of IL-6 between intra-articular injection of LrPRP group and the saline group at the time of day of surgery and day of suture removal when compared with day of evaluation.