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. 2022 Aug 31;12(17):2252.
doi: 10.3390/ani12172252.

Effects of Pelleted and Extruded Feed on Growth Performance, Intestinal Histology and Microbiota of Juvenile Red Swamp Crayfish (Procambarus clarkii)

Jinjuan Wan  1 , Qinkai Xi  2 , Jianqing Tang  1 , Tianji Liu  2 , Cong Liu  2 , Hongqin Li  2 , Xizhang Gu  2 , Meifang Shen  1 , Meiqin Zhang  1 , Jinguang Fang  2 , Xianglong Meng  1
Affiliations

Effects of Pelleted and Extruded Feed on Growth Performance, Intestinal Histology and Microbiota of Juvenile Red Swamp Crayfish (Procambarus clarkii)

Jinjuan Wan et al. Animals (Basel). .

Abstract

The study was conducted to evaluate the extruded and pelleting feed production on growth performance, intestinal histology and microbiome analysis of juvenile red swamp crayfish, Procambarus clarkii. Crayfish were fed either pelleted or extruded feeds that were made using the same formula. Crayfish fed extruded feed had a lower feed conversion ratio, as well as significantly higher levels of trypsin and amylase (p < 0.05) than those fed pelleted feed. However, other growth indices and the activity of lipase were not significantly influenced by the feed processing technique (p > 0.05). In comparison with the pelleted feed group, the lamina propria thickness of crayfish fed extruded feed was significantly lower (p < 0.05). Additionally, the abundance of intestinal microbiota in the extruded feed group was higher than that in the pelleted feed group. The dominant phyla in the intestine of both groups were Proteobacteria, Tenericutes, and Firmicutes, and the relative abundance of Proteobacteria in the extruded feed group was significantly higher than that in the pelleted feed group (p < 0.05). These results revealed that P. clarkii fed extruded feed had higher feed utilization and better intestinal health.

Keywords: aquaculture; digestive enzyme; resident bacteria; xiao-long-xia.

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Conflict of interest statement

Q.X., T.L., C.L., H.L., G.Z. and J.F. are employees of the New Hope Liuhe Co., Ltd. that partly funded our study.

Figures

Figure 1
Figure 1
Histological sections of the intestine of P. clarkii. (a) pelleted feed group, H&E stain (10×); (b) pelleted feed group, PAS stain (10×); (c) extruded feed group, H&E stain (10×); (d) extruded feed group, PAS stain (10×). Scale bar: 100 μm. Uppercase letters indicate examples of measurements of villus height, mucosal fold width, lamina propria thickness and intestinal wall thickness (A, B, C and D, respectively).
Figure 2
Figure 2
(a) Principal coordinate analysis (PCoA) of intestinal bacterial communities of P. clarkii fed pelleted feed and extruded feed; based on weighted UniFrac distances, each point represents a sample, and different colors represent different diets. (b) Venn diagram showing shared and unique OTUs of P. clarkii fed different diets.
Figure 3
Figure 3
Relative abundances of the dominant bacteria at the (a) phylum level and (b) genus level in P. clarkii fed pelleted feed and extruded feed.
See this image and copyright information in PMC

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