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. 2022 Sep 5;19(17):11113.
doi: 10.3390/ijerph191711113.

The Effect of UV Treatment on Surface Contact Angle, Fibroblast Cytotoxicity, and Proliferation with Two Types of Zirconia-Based Ceramics

Vygandas Rutkunas  1 , Rokas Borusevicius  1 , Evaldas Balciunas  2 , Urte Jasinskyte  2 , Milda Alksne  2 , Egidijus Simoliunas  2 , Stefan Zlatev  3 , Vasilena Ivanova  4 , Virginija Bukelskiene  2 , Eitan Mijiritsky  5
Affiliations

The Effect of UV Treatment on Surface Contact Angle, Fibroblast Cytotoxicity, and Proliferation with Two Types of Zirconia-Based Ceramics

Vygandas Rutkunas et al. Int J Environ Res Public Health. .

Abstract

UV photofunctionalization of Zirconia-based materials for abutment fabrication is a promising approach that might influence the formation of a sound peri-implant seal, thus promoting long-term soft and hard tissue implant integration. This study aimed to evaluate the effect of UV treatment of test specimens made by two different ZnO2-based ceramic materials on the hydrophilicity, cell cytotoxicity, and proliferation of human gingival fibroblasts (HGFs). Two Zirconia-based materials, high-translucent and ultra-translucent multi-layered Zirconia (Katana, Kuraray Noritake, Japan), were used to prepare a total of 40 specimens distributed in two equally sized groups based on the material (n = 20). The same surface finishing protocol was applied for all specimens, as suggested by the manufacturer. Half the specimens from each group were treated with UV-C light for 48 h. Water contact angle (WCA), fibroblast cytotoxicity, and proliferation were investigated. The WCA values for the high-translucent Zirconia ranged from 69.9° ± 6.4° to 73.7° ± 13.9° for the treated/non-treated specimens and from 79.5° ± 12.8° to 83.4° ± 11.4° for the ultra-translucent multi-layered Zirconia, respectively. However, the difference was insignificant (F(16) = 3.50, p = 0.292). No significant difference was observed for the fibroblast cytotoxicity test. The results for proliferation revealed a significant difference, which was material-dependent (F(8) = 9.58, p = 0.005). We found that UV surface photofunctionalization of ZrO2-based materials alters the human gingival fibroblast cell viability, which might produce favourable results for cell proliferation.

Keywords: UV photofunctionalization; fibroblast proliferation; surface roughness; water contact angle; zirconia.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Study design scheme.
Figure 2
Figure 2
Dimensions of specimens used in the study.
Figure 3
Figure 3
Profilometry images for both materials included in the study based on the location: (A) ZrO-HT centre, (B) ZrO-HT periphery, (C) ZrO-UTML centre, (D) ZrO-UTML periphery.
Figure 4
Figure 4
Surface roughness of the two materials included in the study.
Figure 5
Figure 5
Cytotoxicity assessment as a ratio of the 12th hour control reference for the four groups included in the study.
Figure 6
Figure 6
The tendency of HGF proliferation in the different study groups for the tested time-points at 24, 48, and 72 h.
See this image and copyright information in PMC

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References

    1. Chien H.-H., Schroering R.L., Prasad H.S., Tatakis D.N. Effects of a New Implant Abutment Design on Peri-Implant Soft Tissues. J. Oral Implantol. 2014;40:581–588. doi: 10.1563/AAID-JOI-D-12-00313. - DOI - PubMed
    1. Al Rezk F., Trimpou G., Lauer H.-C., Weigl P., Krockow N. Response of Soft Tissue to Different Abutment Materials with Different Surface Topographies: A Review of the Literature. Gen. Dent. 2018;66:18–25. - PubMed
    1. Sampatanukul T., Serichetaphongse P., Pimkhaokham A. Histological Evaluations and Inflammatory Responses of Different Dental Implant Abutment Materials: A Human Histology Pilot Study. Clin. Implant Dent. Relat. Res. 2018;20:160–169. doi: 10.1111/cid.12562. - DOI - PubMed
    1. Bharate V., Kumar Y., Koli D., Pruthi G., Jain V. Effect of Different Abutment Materials (Zirconia or Titanium) on the Crestal Bone Height in 1 Year. J. Oral Biol. Craniofacial Res. 2020;10:372–374. doi: 10.1016/j.jobcr.2019.10.001. - DOI - PMC - PubMed
    1. Dede D.Ö., Armağanci A., Ceylan G., Celik E., Cankaya S., Yilmaz B. Influence of Implant Abutment Material on the Color of Different Ceramic Crown Systems. J. Prosthet. Dent. 2016;116:764–769. doi: 10.1016/j.prosdent.2016.04.004. - DOI - PubMed

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