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. 2022 Aug 29;11(17):5070.
doi: 10.3390/jcm11175070.

Growth Factor Release within Liquid and Solid PRF

Katharina Zwittnig  1 , Barbara Kirnbauer  1 , Norbert Jakse  1 , Peter Schlenke  2 , Irene Mischak  1 , Shahram Ghanaati  3 , Sarah Al-Maawi  3 , Dániel Végh  1   4 , Michael Payer  1 , Tomislav A Zrnc  5
Affiliations

Growth Factor Release within Liquid and Solid PRF

Katharina Zwittnig et al. J Clin Med. .

Abstract

Aim: The purpose of this study was to obtain data concerning growth factor release within liquid and solid platelet-rich fibrin (PRF) matrices and to estimate the amount of potential interindividual variations as a basis for further preclinical and clinical trials. Therefore, we aimed to determine possible differences in the release of growth factors between liquid and solid PRF. Materials and Methods: Blood samples obtained from four subjects were processed to both liquid and solid PRF matrices using a standard centrifugation protocol. Five growth factors (vascular endothelial growth factor, VEGF; epidermal growth factor, EGF; platelet-derived growth factor-BB, PDGF-BB; transforming growth factor-β1, TGF-β1; and matrix metallopeptidase 9, MMP-9) have been evaluated at six time points by ELISA over a total observation period of 10 days (1 h, 7 h, 1 d, 2 d, 7 d, and 10 d). Results: Growth factor release could be measured in all samples at each time point. Comparing liquid and solid PRF matrices, no significant differences were detected (p > 0.05). The mean release of VEGF, TGFβ-1, PDGF-BB, and MMP-9 raised to a peak at time point five (day 7) in both liquid and solid PRF matrices. VEGF release was lower in liquid PRF than in solid PRF, whereas those of PDGF-BB and MMP-9 were higher in liquid PRF than in solid PRF at all time points. EGF had its peak release already at time point two after 7 h in liquid and solid matrices (hour 7 EGF solid: mean = 180 pg/mL, SD = 81; EGF liquid: mean = 218 pg/mL, SD = 64), declined rapidly until day 2, and had a second slight peak on day 7 in both groups (day 7 EGF solid: mean = 182 pg/mL, SD = 189; EGF liquid: mean = 81 pg/mL, SD = 70). Conclusions: This study detected growth factor release within liquid and solid PRF matrices with little variations. Further preclinical trials are needed to precisely analyze the growth factor release in larger samples and to better understand their effects on wound healing in different clinical indications.

Keywords: EGF; MMP-9; PDGF-BB; TGF; VEGF; growth factors; oral surgery; platelet-rich fibrin.

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Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

Figures

Figure 1
Figure 1
Growth factor release over time. Growth factor concentrations of liquid (blue) and solid (red) PRF over time for (A) EGF (pg/mL); (B) VEGF (pg/mL); (C) TGFβ-1 (pg/mL); (D) PDGF-BB (pg/mL); and (E) MMP-9 (ng/mL).
Figure 2
Figure 2
Growth factor release with error bars EGF (pg/mL), VEGF (pg/mL), TGFβ-1 (pg/mL), PDGF-BB (pg/mL), and MMP-9 (ng/mL).
See this image and copyright information in PMC

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