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. 2022 Aug 31;27(17):5627.
doi: 10.3390/molecules27175627.

Effects of Extraction Technique on the Content and Antioxidant Activity of Flavonoids from Gossypium Hirsutum linn. Flowers

Jiaxing Dong  1   2 , Kehai Zhou  3 , Xiaoyang Ge  3 , Na Xu  4 , Xiao Wang  1 , Qing He  1   2 , Chenxu Zhang  1   2 , Jun Chu  1   2 , Qinglin Li  1   2
Affiliations

Effects of Extraction Technique on the Content and Antioxidant Activity of Flavonoids from Gossypium Hirsutum linn. Flowers

Jiaxing Dong et al. Molecules. .

Abstract

Cotton is one of the Uyghur medical materials in China and is rich in flavonoids. Flavonoids have important pharmacological effects. The yield of flavonoids in traditional extraction methods is low, which affects the development of flavonoids. Therefore, it is urgent to optimize the extraction techniques. The yield of flavonoids in cotton flowers was effectively improved by response surface methodology, and the highest yield of flavonoids reached 5.66%, and the optimal extraction process conditions were obtained. The DPPH free radical scavenging rate, hydroxyl free radical scavenging rate, superoxide anion free radical scavenging rate, and reducing ability were tested to reflect the antioxidant capacity of flavonoids. The flavonoids had an excellent antioxidant effect. Cell experiments suggested that the flavonoids had the effect of protecting glutamate-induced damage to HT-22 cells. The results of this study provide a theoretical basis for the extraction of cotton flowers flavonoids and the comprehensive evaluation of antioxidant products, as well as the extraction of other plant flavonoids.

Keywords: antioxidant activity; cotton flowers flavonoids; extraction process; response surface methodology.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Figure 1
Figure 1
Analysis of single factor test results of extraction time, liquid ratio, extraction temperature, and ethanol concentration. (a): Effect of extraction time on the extraction rate of flavonoids from cotton flowers; (b): Influence of ratio of liquid to material on extraction rate of flavonoids from cotton flowers; (c): Influence of extraction temperature on extraction rate of flavonoids from cotton flowers; (d): Effect of ethanol concentration on extraction rate of flavonoids from cotton flowers.
Figure 2
Figure 2
Response Surface Methodology Interaction of Four Factors Affecting the Extraction Rate of Flavonoids from Cotton Flowers. (a): The effect of the interaction between liquid-solid ratio and extraction time on the yield of flavonoids. (b): The effect of the interaction of extraction temperature and time on the yield of flavonoids. (c): The effect of the interaction between ethanol concentration and extraction time on the yield of flavonoids. (d): The effect of the interaction between ethanol concentration and liquid-solid ratio on the yield of flavonoids. (e): The effect of the interaction between ethanol concentration and extraction temperature on the yield of flavonoids. (f): The effect of the interaction between ethanol concentration and extraction temperature on the yield of flavonoids. (g): Normal distribution.
Figure 3
Figure 3
Antioxidant activity test result. (a): DPPH free radical scavenging test results. (b): Hydroxyl radical scavenging test results. (c): Results of superoxide anion radical scavenging test. (d): Fe3+ reduction experiments.
Figure 4
Figure 4
The protective effect of flavonoids on HT-22 cells (** p < 0.01, *** p < 0.0001).
See this image and copyright information in PMC

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