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. 2022 Aug 30:2022:1784791.
doi: 10.1155/2022/1784791. eCollection 2022.

The Expression of miR-205 in Prostate Carcinoma and the Relationship with Prognosis in Patients

Zhuifeng Guo  1 , Xuwei Lu  1 , Fan Yang  1 , Liang Qin  1 , Ning Yang  1 , Peiran Cai  2 , Conghui Han  3 , Jiawen Wu  1 , Hang Wang  4
Affiliations

The Expression of miR-205 in Prostate Carcinoma and the Relationship with Prognosis in Patients

Zhuifeng Guo et al. Comput Math Methods Med. .

Retraction in

Abstract

Purpose: We aimed to investigate the changes of serum and cell exosome miR-205 levels in patients with prostate carcinoma and its clinical significance.

Materials and methods: Firstly, pronouncement of miR-205 in normal and prostate carcinoma tissues was analyzed by using UALCAN database. The relationship between miR-205 in tumor tissues and the pathological and clinical characteristics of patients with prostate carcinoma were analyzed. Consequently, 60 people with prostate carcinoma were collected to the Minhang Hospital from August 2016 to August 2021. Serum of patients in the two groups was collected, and RNA in serum exosomes was extracted, and qRT-PCR was used to analyze the expression of miR-205 mediated by serum exosomes. Meanwhile, the relationship among the clinical as well as pathological aspects and bodement of patients with prostate carcinoma and the pronouncement level of miR-205 mediated by exosome was compared. Next, assays like wound healing and CKK-8 were used to investigate the effects of miR-205 in exosomes extracted from prostate carcinoma on the augmentation and metastasis of prostate carcinoma.

Results: The results showed that the pronouncement level of miR-205 in tissues with prostate carcinoma was significantly lower than that in normal prostate tissues. In addition, the pronouncement level of miR-205 in fluid exosome of people with prostate carcinoma and exosomes derived from the lines of prostate carcinoma was considerably less than that in serum exosomes of healthy patients and that of normal cell lines of prostate. The pronouncement level of miR-205 in fluid exosomes of people with prostate carcinoma was negatively associated with cancer phase, uncontrolled cell division in lymph nodes, distant metastasis, and PSA level at initial diagnosis. Analysis (multivariate and univariate) showed that miR-205 pronouncement was a sovereign threat cause for prognosis of prostate cancer patients. Additionally, the pronouncement and metastasis of prostate carcinoma can be restricted by the overexpression of miR-205.

Conclusion: The pronouncement of miR-205 in liquid derived exosomes is correlated with the prediction of people with prostate carcinoma and may be a new marker for identification and cure of prostate carcinoma.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
The miR-205 expression in the database. (a) The miR-205 expression in prostate tumor tissues and normal tissues. (b) The relationship between the expression of miR-205 expression and age in prostate cancer tissues. (c) The relationship between miR-205 expression and lymph node metastasis in prostate cancer tissues. (d) The relationship between miR-205 expression and Gleason score in prostate cancer tissues.
Figure 2
Figure 2
The miR-205 expression in exosomes extracted from prostate carcinoma cell lines and the biological function. (a) The miR-205 expression in exosomes extracted from prostate carcinoma cell lines and normal prostatic cell line of epithelium. (b) The miR-205 expression in exosomes extracted from miR-NC group and miR-205 mimics. (c) The miR-205 promotes the proliferation of prostate cancer cells. (d) The miR-205 promotes the migration of prostate cancer cells. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 (Student's t-test). Abbreviations: HR: hazard ratio; CI: confidence interval.
Figure 3
Figure 3
The relationship between the expression level of serum exosome-mediated miR-205 and pathological, clinical features in prostate cancer patients. (a) The miR-205 expression in serum exosomes of people with prostate tumor and healthy patients. (b) The miR-205 expression was divided into high (above the mean, n = 35) and low (below the mean, n = 25) miR-205 expression classes. (c) The relationship between the expression level of serum exosome-mediated miR-205 and carcinoma stage, lymph node metastasis in prostate carcinoma. (d) The relationship between the expression level of serum exosome-mediated miR-205 and distant metastasis, PSA level in prostate carcinoma at initial diagnosis. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 (Student's t-test).
Figure 4
Figure 4
The relationship between miR-205 expression in serum exosomes of people with prostate cancer and prognosis of patients.
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