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. 1987;9(2-3):687-91.
doi: 10.3109/10641968709164243.

A sensitive method for precise measurement of endogenous angiotensins I, II&III in human plasma

M Kawamura, K Yoshida, S Akabane, Y Matsushima, Y Kawano, S Kojima, N Takahashi, K Shimamoto, K Ito, T Omae

A sensitive method for precise measurement of endogenous angiotensins I, II&III in human plasma

M Kawamura et al. Clin Exp Hypertens A. 1987.

Abstract

We measured endogenous angiotensins (ANGs) I, II&III using a system of extraction by Sep-Pak column followed by high performance liquid chromatography (HPLC) combined with radioimmunoassay (RIA). An excellent separation of ANGs was obtained by HPLC. The recovery of ANGs I, II&III was 80-84%, when these authentic peptides were added to 6 ml of plasma. The coefficient of variation of the ANGs was 0.04-0.09 for intra-assay and 0.08-0.13 for inter-assay, thereby indicating a good reproducibility. Plasma ANGs I, II&III measured by this method in 5 normal volunteers were 51,4.5 and 1.2 pg/ml. In the presence of captopril, ANGs II&III decreased by 84% and 77%, respectively, while ANG I increased 5.1 times. This method is therefore useful to assess the precise levels of plasma ANGs.

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