doi: 10.1093/ajcp/70.4.697.
A reliable method for electron microscopic examination of specific areas from paraffin-embedded tissue mounted on glass slides
- PMID: 360829
- DOI: 10.1093/ajcp/70.4.697
Item in Clipboard
A reliable method for electron microscopic examination of specific areas from paraffin-embedded tissue mounted on glass slides
Am J Clin Pathol.
1978 Oct.
Abstract
A reliable method for electron microscopic examination of specific areas from paraffin-embedded tissue mounted on glass slides. Am J Clin Pathol 70: 697--699, 1978. A method to embed and cut tissue mounted on ordinary glass histologic preparations for electron microscopic observations is described. The cover slip is removed by immersing the slide in xylene for a few minutes to several hours. After the cover slip is removed, the tissue is fixed in osmium tetroxide, dehydrated, and embedded in epoxy resins by inverting a BEEM capsule filled with the resin on a selected area of the slide. After polymerization, thin sections are obtained and observed with the electron microscope with good results.
Similar articles
-
Epoxy-slide embedment of cytochemically stained tissues and cultured cells for light and electron microscopy.Stain Technol. 1986 Jan;61(1):51-8. doi: 10.3109/10520298609110706. Stain Technol. 1986. PMID: 2420040
-
A simple and rapid section embedding technique for sequential light and electron microscopic examination of individually stained central neurons.J Neurosci Methods. 1979 Dec;1(4):383-91. doi: 10.1016/0165-0270(79)90027-x. J Neurosci Methods. 1979. PMID: 397378
-
Agar-screew specimen carrier for bulk processing of biopsy material for electron microscopy.Stain Technol. 1975 Mar;50(2):93-8. doi: 10.3109/10520297509117041. Stain Technol. 1975. PMID: 1096371
-
Embedding method for electron microscopy in biology.Tokai J Exp Clin Med. 1985 Dec;10(6):557-71. Tokai J Exp Clin Med. 1985. PMID: 3915837 Review.
-
Neuropathology for the neuroradiologist: rosettes and pseudorosettes.AJNR Am J Neuroradiol. 2006 Mar;27(3):488-92. AJNR Am J Neuroradiol. 2006. PMID: 16551982 Free PMC article. Review.
Cited by
-
Amyloidosis: What does pathology offer? The evolving field of tissue biopsy.Front Cardiovasc Med. 2022 Dec 5;9:1081098. doi: 10.3389/fcvm.2022.1081098. eCollection 2022. Front Cardiovasc Med. 2022. PMID: 36545023 Free PMC article. Review.
-
Use of the cryostat section in electron microscopy.J Clin Pathol. 1983 Aug;36(8):903-6. doi: 10.1136/jcp.36.8.903. J Clin Pathol. 1983. PMID: 6348101 Free PMC article.
-
Involvement of Fas/Fas ligand in the induction of apoptosis in chronic sialadenitis of minor salivary glands including Sjögren's syndrome.Hum Cell. 2002 Mar;15(1):52-60. doi: 10.1111/j.1749-0774.2002.tb00099.x. Hum Cell. 2002. PMID: 12126064
MeSH terms
LinkOut - more resources
Full Text Sources
