Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Oct;70(4):697-9.
doi: 10.1093/ajcp/70.4.697.

A reliable method for electron microscopic examination of specific areas from paraffin-embedded tissue mounted on glass slides

A Gonzalez-Angulo, I Ruiz de Chavez, M Castaneda

A reliable method for electron microscopic examination of specific areas from paraffin-embedded tissue mounted on glass slides

A Gonzalez-Angulo et al. Am J Clin Pathol. 1978 Oct.

Abstract

A reliable method for electron microscopic examination of specific areas from paraffin-embedded tissue mounted on glass slides. Am J Clin Pathol 70: 697--699, 1978. A method to embed and cut tissue mounted on ordinary glass histologic preparations for electron microscopic observations is described. The cover slip is removed by immersing the slide in xylene for a few minutes to several hours. After the cover slip is removed, the tissue is fixed in osmium tetroxide, dehydrated, and embedded in epoxy resins by inverting a BEEM capsule filled with the resin on a selected area of the slide. After polymerization, thin sections are obtained and observed with the electron microscope with good results.

PubMed Disclaimer

MeSH terms

LinkOut - more resources