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. 2022 Sep 29;387(13):1234-1236.
doi: 10.1056/NEJMc2210648. Epub 2022 Sep 9.

Neutralization of SARS-CoV-2 Omicron BA.2.75 after mRNA-1273 Vaccination

Xiaoying Shen  1 , Spyros Chalkias  2 , Jing Feng  2 , Xing Chen  2 , Honghong Zhou  2 , Jean-Claude Marshall  2 , Bethany Girard  2 , Joanne E Tomassini  2 , Anne Aunins  2 , Rituparna Das  2 , David C Montefiori  3
Affiliations

Neutralization of SARS-CoV-2 Omicron BA.2.75 after mRNA-1273 Vaccination

Xiaoying Shen et al. N Engl J Med. .
No abstract available

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Figures

Figure 1
Figure 1. Neutralization of SARS-CoV-2 D614G and Omicron Variants after mRNA-1273 Booster Dose.
In a phase 2 trial, 20 serum samples were obtained from participants 18 years of age or older (mean age, 55.3) who had received a two-dose primary vaccination series and a single 50-μg booster dose of mRNA-1273 (Table S1 in the Supplementary Appendix). The 50% inhibitory dilution (ID50) geometric mean titers (GMTs) of the samples against ancestral severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) D614G and omicron sublineages (BA.1, BA.2, BA.4 and BA.5 [BA.4/5], and BA.2.75) were assessed at day 29 after administration of the booster dose. The median time from the second dose of primary vaccination to the booster dose was 221 days. Data regarding neutralization of D614G and BA.1 by these samples have been reported previously. The spike mutations of Wuhan-1 (D614G) and the four sublineages of the omicron variant are provided in Figure S1. Mutations in the spike of the BA.2.75 pseudovirus relative to Wuhan-1 are T19I, L24S, P25del, P26del, A27del, G142D, K147E, W152R, F157L, I210V, V213G, G257S, G339H, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, G446S, N460K, S477N, T478K, E484A, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, and N969K. Differences in receptor-binding domain spike mutations of BA.2.75 and BA.4/5 include N460K (present in BA.2.75 and absent in BA.4/5), L452R (present in BA.4/5 and absent in BA.2.75), F486V (present in BA.4/5 and absent in BA.2.75), and G339H in BA.2.75, instead of G339D in BA.4/5. The GMT values relative to D614G and BA.1 for each variant were obtained by first calculating the mean difference in log10 scale; the mean was then back-transformed to the original scale for presentation. The 95% confidence intervals for the GMT reductions were calculated on the basis of the distribution of differences in the log-transformed values and were then back-transformed to the original scale for presentation (Table S2); these 95% confidence intervals are shown as 𝙸 bars in the figure. The circles represent individual samples. The limit of detection (LOD [dashed line]) of the assays was 10. Values below the LOD were assigned a value of 5 for the calculation of summary statistics.
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References

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