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Comment
. 2022 Dec;85(6):702-769.
doi: 10.1016/j.jinf.2022.08.043. Epub 2022 Sep 7.

CRISPR-Cas12a-based detection of monkeypox virus

Yutong Sui  1 , Qi Xu  1 , Mingsheng Liu  1 , Kuiyang Zuo  1 , Xiaomei Liu  2 , Jinyu Liu  3
Affiliations
Comment

CRISPR-Cas12a-based detection of monkeypox virus

Yutong Sui et al. J Infect. 2022 Dec.
No abstract available

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Conflict of interest statement

Declaration of competing interest The authors declare no conflict of interests.

Figures

Fig 1
Fig. 1
The CRISPR-Cas12a-based system for detection of monkeypox virus. (A) Schematic of CRISPR-Cas12a-based system proposed in this study. (B) Fluorescence signal of CRISPR-Cas12a-based assay for target DNA saturates within 10 min. (C and D) The fluorescence intensity (λem = 520 nm) for the detection of the monkeypox viral F3L (C) and N3R (D) genes at various concentrations. The assay revealed a statistically significant detection of F3L and N3R DNAs, at a concentration as low as 2 fM (⁎⁎P < 0.01 vs. no RNA).
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