Proteomic analysis reveals molecular mechanism of Cd2+ tolerance in the leaves of halophyte Halogeton glomeratus

Abstract

Halogeton glomeratus (H. glomeratus) is categorized as a halophyte, it can potentially endure not only salt but also heavy metals. The aim of this work was to study the molecular mechanisms underlying the Cd²⁺ tolerance of halophyte H. glomeratus seedlings. For that we used a combination of physiological characteristics and data-independent acquisition-based proteomic approaches. The results revealed that the significant changes of physiological characteristics of H. glomeratus occurred under approximately 0.4 mM Cd²⁺ condition and that Cd²⁺ accumulated in Cd²⁺-treated seedling roots, stems and leaves. At the early stage of Cd²⁺ stress, numerous differentially abundant proteins related to "phosphoenolpyruvate carboxylase", "transmembrane transporters", and "vacuolar protein sorting-associated protein" took important roles in the response of H. glomeratus to Cd²⁺ stress. At the later stage of Cd²⁺ stress, some differentially abundant proteins involved in "alcohol-forming fatty acyl-CoA reductase", "glutathione transferase", and "abscisic acid receptor" were considered to regulate the adaptation of H. glomeratus exposed to Cd²⁺ stress. Finally, we found various detoxification-related differentially abundant proteins related to Cd²⁺ stress. These biological processes and regulators synergistically regulated the Cd²⁺ tolerance of H. glomeratus. SIGNIFICANCE: The halophyte, H.glomeratus, has a strong tolerance to salinity, also survives in the heavy metal stress. At present, there are few reports on the comprehensive characterization and identification of Cd²⁺ response and adaption related regulators in H.glomeratus. This research focuses on the molecular mechanisms of H. glomeratus tolerance to Cd²⁺ stress at proteome levels to uncover the novel insight of the Cd²⁺-related biological processes and potential candidates involved in the response and adaption mechanism. The results will help elucidate the genetic basis of this species' tolerance to Cd²⁺ stress and develop application prospect of wild genetic resources to heavy metal phytoremediation.

Keywords: Biological processes; Data-independent acquisition; Differentially abundant proteins; Halogeton glomeratus; Molecular mechanisms.