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. 2022:2574:209-219.
doi: 10.1007/978-1-0716-2712-9_9.

T-Cell Repertoire Characterization

Anna Pasetto  1 , Marcus Buggert  2
Affiliations

T-Cell Repertoire Characterization

Anna Pasetto et al. Methods Mol Biol. 2022.

Abstract

T-cell repertoire characterization is a methodology that enables the identification of T-cell receptor (TCR) gene sequences in a T-cell population. TCR genes are composed of modular gene segments V (D) J that undergo somatic recombination, resulting in unique and unpredictable sequences that can be utilized to identify each T-cell clone. The analysis of the TCR composition in a T-cell population can give information on the biological phenomenon such as antigen-driven expansion and heterogeneity of T-cell responses. Bulk TCR analysis can give useful information on the clonality and can help track a specific clonotype over time or in different compartments, although the information about pairing cannot be provided. Single-cell TCR sequencing, on the other hand, can provide pairing information that are necessary to reconstruct the TCR and confirm antigen specificity.Here we describe common methods to characterize T-cell repertoires based on both bulk and single-cell next-generation sequencing.

Keywords: Next generation sequencing; Single cell; T-cell receptor.

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References

    1. Turner SJ et al (2006) Structural determinants of T-cell receptor bias in immunity. Nat Rev Immunol 6(12):883–894 - DOI
    1. Pasetto A, Lu YC (2021) Single-cell TCR and transcriptome analysis: an indispensable tool for studying T-cell biology and cancer immunotherapy. Front Immunol 12:689091 - DOI
    1. Kim SM et al (2012) Analysis of the paired TCR alpha- and beta-chains of single human T cells. PLoS One 7(5):e37338 - DOI
    1. Pasetto A et al (2016) Tumor- and neoantigen-reactive T-cell receptors can be identified based on their frequency in fresh tumor. Cancer Immunol Res 4(9):734–743 - DOI
    1. Paria BC et al (2021) Rapid identification and evaluation of neoantigen-reactive T-cell receptors from single cells. J Immunother 44(1):1–8 - DOI

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