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. 2022 Sep:89:106153.
doi: 10.1016/j.ultsonch.2022.106153. Epub 2022 Sep 7.

Effects of ultrasonic treatment on ovomucin: Structure, functional properties and bioactivity

Qi Xu  1 , Xuanchen Li  2 , Yunzheng Lv  2 , Yaping Liu  3 , Chunfang Yin  2
Affiliations

Effects of ultrasonic treatment on ovomucin: Structure, functional properties and bioactivity

Qi Xu et al. Ultrason Sonochem. 2022 Sep.

Abstract

The effects of ultrasonic treatment on the structure, functional properties and bioactivity of Ovomucin (OVM) were investigated in this study. Ultrasonic treatment could significantly enhance OVM solubility without destroying protein molecules. The secondary structure changes, including β-sheet reduction and random coil increase, indicate more disorder in OVM structure. After ultrasonic treatment, the OVM molecule was unfolded partially, resulting in the exposure of hydrophobic regions. The changes in OVM molecules led to an increase in intrinsic fluorescence and surface hydrophobicity. By detecting the particle size of protein solution, it was confirmed that ultrasonic treatment disassembled the OVM aggregations causing a smaller particle size. Field emission scanning electron microscopy (FE-SEM) images showed that ultrasonic cavitation significantly reduced the tendency of OVM to form stacked lamellar structure. Those changes in structure resulted in the improvement of foaming, emulsification and antioxidant capacity of OVM. Meanwhile, the detection results of ELISA showed that ultrasonic treatment did not change the biological activity of OVM. These results suggested that the relatively gentle ultrasound treatment could be utilized as a potential approach to modify OVM for property improvement.

Keywords: Bioactivity; Function properties; Ovomucin; Solubility; Ultrasound.

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Conflict of interest statement

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
Solubility in water of untreated and sonicated OVM. Different superscript letters in the figure denote significant differences (p < 0.05).
Fig. 2
Fig. 2
Effects of ultrasound treatment on the molecular weight of OVM. SDS-PAGE (A) and Native-PAGE (B).
Fig. 3
Fig. 3
Effects of ultrasonic treatment on surface hydrophobicity (A) and intrinsic fluorescence (B) of OVM molecular.
Fig. 4
Fig. 4
Effects of ultrasonic treatment on average particle size (A) and secondary structure (B) of OVM molecule. Different superscript letters in the figure denote significant differences (p < 0.05).
Fig. 5
Fig. 5
Morphology of OVM determined by scanning electron microscopy. Untreated (A); 10 min (B); 20 min (C); 30 min (D); 40 min (E).
Fig. 6
Fig. 6
Effects of ultrasonic treatment on foam properties of OVM. Foam expansion (A) and foam stability (B). Different superscript letters in the figure denote significant differences (p < 0.05).
Fig. 7
Fig. 7
Effects of ultrasonic treatment on emulsion properties of OVM. Emulsion activity index (A) and emulsion stability index (B). Different superscript letters in the figure denote significant differences (p < 0.05).
Fig. 8
Fig. 8
Changes in antioxidant activity of OVM after different ultrasonic treatment time. ABTS scavenging rate (A) and DPPH scavenging rate (B). Different superscript letters in the figure denote significant differences (p < 0.05).
Fig. 9
Fig. 9
Antibody binding ability of OVM after ultrasonic treatment with different times (p > 0.05).
See this image and copyright information in PMC

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