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. 2022 Aug 25;7(35):30794-30800.
doi: 10.1021/acsomega.2c02064. eCollection 2022 Sep 6.

Effect of the Graphene- Ni/NiFe2O4 Composite on Bacterial Inhibition Mediated by Protein Degradation

Narayanam Phani Satyanarayana Acharyulu  1   2 , Arya Sohan  3 , Pravallika Banoth  3 , Srinivasu Chintalapati  4 , Sejal Doshi  5 , Venu Reddy  6   7 , Chella Santhosh  8 , Andrews Nirmala Grace  9 , Luis De Los Santos Valladares  10   11 , Pratap Kollu  3
Affiliations

Effect of the Graphene- Ni/NiFe2O4 Composite on Bacterial Inhibition Mediated by Protein Degradation

Narayanam Phani Satyanarayana Acharyulu et al. ACS Omega. .

Abstract

Recent investigations have demonstrated that nickel ferrite nanoparticles and their derivatives have toxicity effects on bacterial cells. In this study, we have prepared nickel ferrite nanoparticles (Ni/NiFe2O4) and nickel/nickel ferrite graphene oxide (Ni/NiFe2O4-GO) nanocomposite and evaluated their toxic effects on E. coli cells ATCC 25922. The prepared nanomaterials were characterized using X-ray diffraction, Raman spectroscopy, scanning electron microscopy, transmission electron microscopy, and vibrating sample magnetometry techniques. The toxicity was evaluated using variations in cell viability, cell morphology, protein degradation, and oxidative stress. Ni/NiFe2O4-GO nanocomposites likewise prompt oxidative stress proved by the age of reactive oxygen species (ROS) and exhaustion of antioxidant glutathione. This is the first report indicating that Ni/NiFe2O4-GO nanocomposite-initiated cell death in E. coli through ROS age and oxidative stress.

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Conflict of interest statement

The authors declare no competing financial interest.

Figures

Figure 1
Figure 1
XRD pattern of the Ni/NiFe2O4–GO nanocomposite. Inset: Williamson–Hall plot. “Reprinted XRD graph with permission from [Applied Physics Letters, https://doi.org/10.1063/1.4892476]. Copyright [2014] [AIP Publishing].”
Figure 2
Figure 2
FE-SEM images of Ni/NiFe2O4–GO at various magnifications.
Figure 3
Figure 3
HR-TEM images of Ni/NiFe2O4–GO with various magnifications.
Figure 4
Figure 4
Raman Spectra of Ni/NiFe2O4–GO nanocomposite.
Figure 5
Figure 5
Cell viability of E. coli when treated with GO, NiFe2O4, and Ni/NiFe2O4–GO. (a) concentration-dependent study of 0–200 ppm reduced graphene oxide-Ni/NiFe2O4 with E. coli cells. (b) time-dependent (0–90 min) study of reduced graphene oxide-Ni/NiFe2O4 treated with E. coli cells.
Figure 6
Figure 6
FEG-SEM images of (a) living E. coli and (b) its destruction after treatment with Ni/NiFe2O4–GO.
Figure 7
Figure 7
Protein degradation by graphene oxide (GO) and Ni/NiFe2O4–GO.
Figure 8
Figure 8
Oxidative stress release by E. coli because of the effect of the reduced graphene oxide- Ni/NiFe2O4 composite.
See this image and copyright information in PMC

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