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. 2022 Sep-Oct;36(5):2414-2421.
doi: 10.21873/invivo.12975.

Evaluation of CD4+:CD8+ Ratio in Patients With Cervical Cancer and the Levels of Inflammatory Markers

Magdi M Salih  1 , Mazen Almehmadi  2 , Alaa Shafie  2 , Abdulaziz Alsharif  2 , Naif Alsiwiehri  2 , Ahmad El-Askary  2 , Khalid Alzahrani  2 , Abdulelah Aljuaid  2 , Osama Abdulaziz  2 , Amani Ahmed Alrehaili  2 , Abdulraheem A Almalki  2 , Hassan Swed Alzahrani  3 , Mustafa Halawi  4 , Safar Almalki  3 , Ebtisam Alosimi  2 , Amal F Gharib  2
Affiliations

Evaluation of CD4+:CD8+ Ratio in Patients With Cervical Cancer and the Levels of Inflammatory Markers

Magdi M Salih et al. In Vivo. 2022 Sep-Oct.

Abstract

Background/aim: Cervical cancer remains a major public health concern. The ratio of CD4+:CD8+ T-cells is used to evaluate the immune system function. This study aimed to explore the CD4+:CD8+ T-cell ratio in relation to the glycemic status, inflammatory markers, vitamin D, and vitamin B12 in patients with early diagnosed cervical cancer.

Patients and methods: This is a cross-sectional study. Blood samples were collected for flow cytometry analysis. Information regarding Papanicolaou (Pap) smears and colposcopy investigations were collected from 152 women with type 2 diabetes admitted to East Jeddah Hospital, Jeddah, Saudi Arabia, between January 2018 and January 2021.

Results: Patients with early cervical carcinoma and a higher CD4+:CD8+ ratio (>1.2) had a higher C-reactive protein (CRP) level than those with a lower CD4+:CD8+ ratio (Mean±SD=13.75±13.3 vs. 10.85±8.1; p-value=0.034). Patients with early cervical carcinoma, diabetes, and higher CD4+:CD8+ ratio (>1.2) had a higher blood HbA1c percent than those with a lower CD4+:CD8+ ratio.

Conclusion: A high CD4+:CD8+ T-cells ratio was associated with an increased HbA1c% and CRP levels in women with diabetes diagnosed with early cervical carcinoma, which can induce inflammation in early diagnosed patients with cervical cancer.

Keywords: C-Reactive protein; Cervical cancer; diabetic women; early cervical carcinoma.

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Conflict of interest statement

The Authors declare no conflicts of interest in relation to this study.

Figures

Figure 1
Figure 1. Assessment of CD4+:CD8+ ratio. FACS canto was used for evaluation of CD4+:CD8+ ratio. The current patient has 3,261 CD4+ cells/mm3 and 1,811 CD8+cells/mm3, which results in a ratio of 1.8.
Figure 2
Figure 2. Correlation of CD4+:CD8+ ratio with inflammatory markers. Scatter plots were used to study the correlation between the ratio of CD4+:CD8+ lymphocytes and inflammatory markers. HBA1C, CRP, ferritin, and Vitamin B12 levels show positive correlation with the increase of the ratio of CD4+:CD8+ lymphocytes. While D-dimer and Vitamin D show negative correlation with the ratio of CD4+:CD8+ lymphocytes. CRP, and D-dimer show linear correlation, and HBA1C shows slightly positive correlation.
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