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. 2022 Sep-Oct;36(5):2414-2421.
doi: 10.21873/invivo.12975.

Evaluation of CD4+:CD8+ Ratio in Patients With Cervical Cancer and the Levels of Inflammatory Markers

Magdi M Salih  1 , Mazen Almehmadi  2 , Alaa Shafie  2 , Abdulaziz Alsharif  2 , Naif Alsiwiehri  2 , Ahmad El-Askary  2 , Khalid Alzahrani  2 , Abdulelah Aljuaid  2 , Osama Abdulaziz  2 , Amani Ahmed Alrehaili  2 , Abdulraheem A Almalki  2 , Hassan Swed Alzahrani  3 , Mustafa Halawi  4 , Safar Almalki  3 , Ebtisam Alosimi  2 , Amal F Gharib  2
Affiliations

Evaluation of CD4+:CD8+ Ratio in Patients With Cervical Cancer and the Levels of Inflammatory Markers

Magdi M Salih et al. In Vivo. 2022 Sep-Oct.

Abstract

Background/aim: Cervical cancer remains a major public health concern. The ratio of CD4+:CD8+ T-cells is used to evaluate the immune system function. This study aimed to explore the CD4+:CD8+ T-cell ratio in relation to the glycemic status, inflammatory markers, vitamin D, and vitamin B12 in patients with early diagnosed cervical cancer.

Patients and methods: This is a cross-sectional study. Blood samples were collected for flow cytometry analysis. Information regarding Papanicolaou (Pap) smears and colposcopy investigations were collected from 152 women with type 2 diabetes admitted to East Jeddah Hospital, Jeddah, Saudi Arabia, between January 2018 and January 2021.

Results: Patients with early cervical carcinoma and a higher CD4+:CD8+ ratio (>1.2) had a higher C-reactive protein (CRP) level than those with a lower CD4+:CD8+ ratio (Mean±SD=13.75±13.3 vs. 10.85±8.1; p-value=0.034). Patients with early cervical carcinoma, diabetes, and higher CD4+:CD8+ ratio (>1.2) had a higher blood HbA1c percent than those with a lower CD4+:CD8+ ratio.

Conclusion: A high CD4+:CD8+ T-cells ratio was associated with an increased HbA1c% and CRP levels in women with diabetes diagnosed with early cervical carcinoma, which can induce inflammation in early diagnosed patients with cervical cancer.

Keywords: C-Reactive protein; Cervical cancer; diabetic women; early cervical carcinoma.

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Conflict of interest statement

The Authors declare no conflicts of interest in relation to this study.

Figures

Figure 1
Figure 1. Assessment of CD4+:CD8+ ratio. FACS canto was used for evaluation of CD4+:CD8+ ratio. The current patient has 3,261 CD4+ cells/mm3 and 1,811 CD8+cells/mm3, which results in a ratio of 1.8.
Figure 2
Figure 2. Correlation of CD4+:CD8+ ratio with inflammatory markers. Scatter plots were used to study the correlation between the ratio of CD4+:CD8+ lymphocytes and inflammatory markers. HBA1C, CRP, ferritin, and Vitamin B12 levels show positive correlation with the increase of the ratio of CD4+:CD8+ lymphocytes. While D-dimer and Vitamin D show negative correlation with the ratio of CD4+:CD8+ lymphocytes. CRP, and D-dimer show linear correlation, and HBA1C shows slightly positive correlation.
See this image and copyright information in PMC

References

    1. Arbyn M, Weiderpass E, Bruni L, de Sanjosé S, Saraiya M, Ferlay J, Bray F. Estimates of incidence and mortality of cervical cancer in 2018: a worldwide analysis. Lancet Glob Health. 2020;8(2):e191–e203. doi: 10.1016/S2214-109X(19)30482-6. - DOI - PMC - PubMed
    1. de Sanjose S, Quint WG, Alemany L, Geraets DT, Klaustermeier JE, Lloveras B, Tous S, Felix A, Bravo LE, Shin HR, Vallejos CS, de Ruiz PA, Lima MA, Guimera N, Clavero O, Alejo M, Llombart-Bosch A, Cheng-Yang C, Tatti SA, Kasamatsu E, Iljazovic E, Odida M, Prado R, Seoud M, Grce M, Usubutun A, Jain A, Suarez GA, Lombardi LE, Banjo A, Menéndez C, Domingo EJ, Velasco J, Nessa A, Chichareon SC, Qiao YL, Lerma E, Garland SM, Sasagawa T, Ferrera A, Hammouda D, Mariani L, Pelayo A, Steiner I, Oliva E, Meijer CJ, Al-Jassar WF, Cruz E, Wright TC, Puras A, Llave CL, Tzardi M, Agorastos T, Garcia-Barriola V, Clavel C, Ordi J, Andújar M, Castellsagué X, Sánchez GI, Nowakowski AM, Bornstein J, Muñoz N, Bosch FX, Retrospective International Survey and HPV Time Trends Study Group Human papillomavirus genotype attribution in invasive cervical cancer: a retrospective cross-sectional worldwide study. Lancet Oncol. 2010;11(11):1048–1056. doi: 10.1016/S1470-2045(10)70230-8. - DOI - PubMed
    1. Li J, Wu MF, Lu HW, Zhang BZ, Wang LJ, Lin ZQ. Impact of hyperglycemia on outcomes among patients receiving neoadjuvant chemotherapy for bulky early stage cervical cancer. PLoS One. 2016;11(11):e0166612. doi: 10.1371/journal.pone.0166612. - DOI - PMC - PubMed
    1. Chen S, Tao M, Zhao L, Zhang X. The association between diabetes/hyperglycemia and the prognosis of cervical cancer patients: A systematic review and meta-analysis. Medicine (Baltimore) 2017;96(40):e7981. doi: 10.1097/MD.0000000000007981. - DOI - PMC - PubMed
    1. Shah W, Yan X, Jing L, Zhou Y, Chen H, Wang Y. A reversed CD4/CD8 ratio of tumor-infiltrating lymphocytes and a high percentage of CD4(+)FOXP3(+) regulatory T cells are significantly associated with clinical outcome in squamous cell carcinoma of the cervix. Cell Mol Immunol. 2011;8(1):59–66. doi: 10.1038/cmi.2010.56. - DOI - PMC - PubMed

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