Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Sep;2(9):e549.
doi: 10.1002/cpz1.549.

Immunohistochemistry

Patricia A Loughran  1   2 , Mark A Ross  1 , Claudette M St Croix  1
Affiliations

Immunohistochemistry

Patricia A Loughran et al. Curr Protoc. 2022 Sep.

Abstract

Immunohistochemistry is an essential technique for the localization and measurement of proteins in cells and tissues. This article describes methods for labeling proteins in adherent and suspension cell cultures and in tissue sections. Choices of antibodies and detection methods are discussed, and detailed troubleshooting guidelines are provided. © 2022 Wiley Periodicals LLC. Basic Protocol 1: Immunofluorescent labeling of cells grown as adherent monolayers Alternate Protocol 1: Immunofluorescent labeling of cells in suspension Basic Protocol 2: Immunofluorescent labeling of tissue sections Alternate Protocol 2: Immunofluorescent labeling using streptavidin-biotin conjugates Alternate Protocol 3: Immunofluorescent double-labeling of tissue sections Alternate Protocol 4: Immunofluorescent double-labeling of tissue sections with two primary antibodies from the same host species.

Keywords: antibodies and fluorophore; imaging; immunofluorescence; microscopy.

PubMed Disclaimer

References

Literature Cited

    1. Coons, A. H., Creech, H. J., & Jones, R. N. (1941). Immunological properties of an antibody containing a fluorescent group. Proceedings of the Society for Experimental Biology and Medicine, 47, 200-202. doi: 10.3181/00379727-47-13084P
    1. Ross, M. A., Kohut, L., & Loughran, P. A. (2022). Cryosectioning. Current Protocols, 2(1), e342. doi: 10.1002/cpz1.342
    1. Watkins, S. (2009). Immunohistochemistry. Current Protocols in Cytometry, 48, 12.16.1-12.16.10. doi: 10.1002/0471142956.cy1216s48

LinkOut - more resources