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. 2022 Dec;49(12):11601-11609.
doi: 10.1007/s11033-022-07876-2. Epub 2022 Sep 14.

Inheritance of androgenesis response in pepper

Nihal Denli  1 , Atilla Ata  2 , Davut Keleş  2 , Nedim Mutlu  3 , Hatıra Taşkın  4
Affiliations

Inheritance of androgenesis response in pepper

Nihal Denli et al. Mol Biol Rep. 2022 Dec.

Abstract

Background: Anther culture has become an important part of pepper breeding. Response to haploidy via androgenesis is highly genotype-specific. However, studies on the inheritance of response to anther culture are lacking. Therefore, the study aimed to determine the inheritance of androgenesis.

Methods and results: The plant material included crosses involving Capsicum annuum L. (253 A, and Inan3363) X C. chinense PI 159,236 populations. To estimate the heritability of the trait, two pepper lines with very high androgenesis responses were crossed with PI 159,236, a non-responsive accession. The androgenesis response was phenotyped using the parents, F1, F2, BC1P1, and BC1P2/P3 created through reciprocal crosses. Using variance components, the number of genes controlling the trait, broad (H2 = 0.97), and narrow sense heritabilities (h2 = 0.20), genetic variance (VG=113.8), the additive (VA=23.9), and the dominance gene variances (VD = 89.9), as well as the environmental variance (VE = 3.6) were calculated. Additive and dominant gene effects were 21% and 79%, respectively. Results derived from two different populations showed that the number of genes controlling the trait was between 1.96 and 2.46, and H2 = 0.96-0.97, h2 = 0.20-0.65, VG=91.8-113.8, VA, = 23.9-62.7, VD = 29.1-89.9, and VE=3.5-3.6 were calculated. The X2 analysis indicated that the most suitable one is the 9: 3: 4 epistatic genetic model (X2 = 2.13, P = 0.343, N = 155).

Conclusions: Results obtained from two different populations indicate the existence of a few major genes for response to androgenesis in pepper. Elucidating the inheritance of androgenesis is expected to pave the way for tagging the gene(s) in the pepper genome.

Keywords: Androgenic response; C. annuum; C. chinense; Inheritance.

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