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. 2022 Nov;8(6):2538-2544.
doi: 10.1002/vms3.921. Epub 2022 Sep 14.

Improving obesity and lipid metabolism using conjugated linoleic acid

Ye Sun  1 , Xufeng Hou  2 , Lingjie Li  2 , Yanqing Tang  2 , Mingyue Zheng  2 , Weisen Zeng  2 , XiaoLong Lei  3
Affiliations

Improving obesity and lipid metabolism using conjugated linoleic acid

Ye Sun et al. Vet Med Sci. 2022 Nov.

Abstract

Background: Conjugated linoleic acid (CLA) can prevent fatty acid accumulation induced by a high-fructose diet and improve lipid metabolism disorders in patients.

Objectives: We aimed to investigate the effect of CLA on obesity and lipid metabolism and its possible mechanism.

Methods: Eight-month-old male BKS.Cg-Dock7m +/+ Leprdb /JNju (db/db) mice (n = 12) were fed a CLA mix composed of equivalent c9, t11-CLA and t10, c12-CLA for 1 month. The effect of CLA on body weight, water and food intake, and triglyceride (TG) and total cholesterol (TC) levels was investigated. PPARα, PPARγ and CD36 expression was determined by quantitative PCR and western blotting. Additionally, the expression of these three genes was studied in HepG2 cells treated with CLA and linoleic acid.

Results: CLA treatment notably reduced the dietary and water intake of db/db mice, effectively reduced body weight, and decreased serum TG and TC levels (p < 0.05). Increased expression of PPARα (p < 0.05) and decreased expression of CD36 (p < 0.001) were observed in the liver of mice that were fed CLA. CLA increased PPARα expression (p < 0.001) and decreased PPARγ (p < 0.001) and CD36 expression (p < 0.01) in HepG2 cells.

Conclusions: Our results showed that CLA can improve lipid metabolism in obese mice through upregulation of PPARα expression and downregulation of CD36 expression.

Keywords: lipid metabolism; nutrition; obesity.

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Conflict of interest statement

The authors declare no potential conflicts of interests.

Figures

FIGURE 1
FIGURE 1
Water and food intake amount of the control and CLA groups. (a) Water intake amount (g). (b) Food intake amount (g)
FIGURE 2
FIGURE 2
Body weight changes (g) in the control and CLA groups.
FIGURE 3
FIGURE 3
Serum TG and TC levels in the control and CLA groups. ***p < 0.001
FIGURE 4
FIGURE 4
Comparison of expression levels of PPARγ, PPARɑ and CD36 genes in the control and CLA groups. (a) Detection of mRNA expression of the three genes in the two groups qRT‐PCR. (b) Determination of protein expression quantity of the three genes in the two groups by western blotting. *p < 0.05, ***p < 0.001
FIGURE 5
FIGURE 5
mRNA and protein expression levels of CD36, PPARα and PPARγ after treatment with linoleic acid, c‐9, t11‐CLA, or t10, c12‐CLA. (a)–(c) qRT‐PCR results. (d) Western blotting results. **p < 0.01, ***p < 0.001
FIGURE 6
FIGURE 6
(a) mRNA expression level of PPARγ is significantly decreased after gene knockdown. (b) The protein expression level of PPARγ is significantly decreased after gene knockdown. (c) CD36 protein expression is significantly decreased after PPARγ gene knockdown. ***p < 0.001
FIGURE 7
FIGURE 7
Expression of BODIPYTM FL C16, PPARαRNAi and CD36 using a laser confocal microscope (600×)
See this image and copyright information in PMC

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