doi: 10.1007/BF00518727.
An improved procedure for the X-ray microanalysis of acid phosphatase activity in lysosomes
- PMID: 3610675
- DOI: 10.1007/BF00518727
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An improved procedure for the X-ray microanalysis of acid phosphatase activity in lysosomes
Histochemistry.
1987.
Abstract
The useful detection of acid phosphatase activity with cerium as a capturing agent is confirmed. By introducing a freeze step in combination with a preincubation, reliably localized, lysosomal precipitates are obtained and aspecific ones prevented. Short (t less than 1 h) postfixation with either OsO4 plus K4Fe (CN)6 or OsO4 plus aminotriazole, added to lysosomal cerium localization a high membrane contrast. The detection of cerium by X-ray microanalysis is improved by a better spectral separation of the osmium (M alpha) and cerium (L alpha) peaks.
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