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. 2023 Mar 30;27(1):49-54.
doi: 10.5935/1518-0557.20220037.

Serum progesterone level in luteal phase improves pregnancy rate in fresh cycles with blastocyst embryo transfer

Juliano Brum Scheffer  1 , Bruno Brum Scheffer  1 , Ana Paula de Souza Aguiar  1 , Juliana Baumgratz Franca  1 , Daniel Mendez Lozano  2 , Renato Fanchin  3
Affiliations

Serum progesterone level in luteal phase improves pregnancy rate in fresh cycles with blastocyst embryo transfer

Juliano Brum Scheffer et al. JBRA Assist Reprod. .

Abstract

Objective: To assess the association between serum level of progesterone during stimulation and in the luteal phase with pregnancy rate in a cohort of patients undergoing in vitro fertilization and embryo transfer (IVF-ET) on day 5.

Methods: Retrospective Cohort Study. Patients: 62 infertile women, aged 24-42 years, undergoing ART at our center from May 2019 to May 2021. Progesterone was evaluated during ovarian stimulation on Day 2, Day 6, and Day 8 of stimulation, day of trigger (P4dhCG), and on the day of blastocyst transfer with 5 days of progesterone supplementation (P4d5+). We also calculated the difference of P4d5+ with P4dhCG. (∆P4). Then we divided the patients into two groups based on progesterone serum levels at P4d5+; <10ng/ml (Group A), ≥10ng/ml (Group B). The Student's t-test was performed for continuous variables; Mann-Whitney's Test and Spearman's Test were used where appropriate for categorical variables. p<0.05 was considered statistically significant.

Results: There were positive correlations between βhCG positive with P4d5+ (p<0.001; Rho 0.770) and ∆P4 (p<0.001; Rho 0.703). The pregnancy rate doubled when the serum progesterone level was ≥10ng/ml on the fifth day of progesterone supplementation compared with P4<10ng/ml (44% vs. 21%, respectively).

Conclusions: The pregnancy rate was positively correlated with the serum P4 level on the fifth day of progesterone supplementation and with the difference between the serum progesterone level in the Dd5+ / dhCG. A higher pregnancy rate was observed when serum progesterone level on the fifth day of progesterone supplementation was ≥10ng/ml.

Keywords: ART; luteal phase; pregnancy rate; progesterone.

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Figures

Figure 1
Figure 1
Correlation between a positive βhCG level and P4d5+ (p<0.001; Rho 0.770).
Figure 2
Figure 2
Correlation between βhCG positive with ∆P4 (p<0,001; Rho 0.703).
See this image and copyright information in PMC

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