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. 2023:2564:121-141.
doi: 10.1007/978-1-0716-2667-2_6.

Development and Characterization of Flavin-Binding Fluorescent Proteins, Part I: Basic Characterization

Alina Remeeva  1 , Anna Yudenko  1 , Vera V Nazarenko  1 , Oleg Semenov  1 , Anastasia Smolentseva  1 , Andrey Bogorodskiy  1 , Ivan Maslov  1 , Valentin Borshchevskiy  1 , Ivan Gushchin  2
Affiliations

Development and Characterization of Flavin-Binding Fluorescent Proteins, Part I: Basic Characterization

Alina Remeeva et al. Methods Mol Biol. 2023.

Abstract

Flavin-based fluorescent proteins (FbFPs) are small fluorescent proteins derived from light-oxygen-voltage (LOV) domains. The proteins bind ubiquitous endogenous flavins as chromophores and can be used as versatile in vivo reporter proteins under aerobic and anaerobic conditions. This chapter presents the methodology to identify LOV domain sequences in genomic databases; design new FbFPs; characterize their biochemical, spectroscopic, photophysical, and photochemical properties; and conduct basic fluorescence microscopy experiments.

Keywords: Flavin; Flavin-binding fluorescent protein; Fluorescence microscopy; Genome mining; LOV domain.

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