doi: 10.1016/0005-2787(78)90227-7.
Effect of DNA denaturants on the lac repressor-operator interaction
- PMID: 361087
- DOI: 10.1016/0005-2787(78)90227-7
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Effect of DNA denaturants on the lac repressor-operator interaction
Biochim Biophys Acta.
.
Abstract
The nitrocellulose filter assay was used to study the effect of the DNA denaturants glycerol and dimethylsulfoxide (Me2SO) on the lac repressor-operator interaction. Both glycerol and Me2SO decrease the rate of dissociation (kb) of the repressor-operator complex but do not significantly alter the rate of association of repressor and operator. In the presence of 10% Me2SO an almost 10-fold increase of affinity of repressor for operator is observed. A small increase in affinity of repressor for Escherichia coli DNA, chicken blood DNA, and poly(dA-dT) is also found. The results lead to the conclusion that lac repressor when interacting with the operator causes local destabilization of the DNA.
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