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. 2022 Jul;14(Suppl 1):S344-S351.
doi: 10.4103/jpbs.jpbs_47_22. Epub 2022 Jul 13.

Evaluation of Salivary and Plasma Levels of Salivary α-Amylase in Patients of Oral Squamous Cell Carcinoma

Abhishek  1 , Vaibhava Raaj  1 , Sakshi Raina  2 , Anusha  1 , Nikhil Raj  1 , Rajat Sehgal  3
Affiliations

Evaluation of Salivary and Plasma Levels of Salivary α-Amylase in Patients of Oral Squamous Cell Carcinoma

Abhishek et al. J Pharm Bioallied Sci. 2022 Jul.

Abstract

Aim: The aim of the study was to evaluate levels of salivary alpha-amylase (sAA) in plasma and saliva of patients with oral squamous cell carcinoma (SCC). The diurnal patterns of sAA and its response to stress were also determined.

Materials and methods: A randomized clinical study was conducted to evaluate the salivary and plasma levels of sAA in three study groups, containing ten subjects each. sAA concentration in plasma and saliva samples was measured using Bioassay Technology Laboratory human alpha-amylase kits, and the levels were compared among control and test groups.

Results: In all groups, the mean plasma α-amylase level and mean saliva α-amylase level show an increasing trend with time, i.e., from morning to night, and among the groups, it was highest in Group III followed by Group II and Group I the least (Group III, Group II, and Group I) at all times with significantly higher mean values in Group III subjects at all times of a day.

Conclusion: Alpha-amylase is one of the principal salivary proteins and its secretion is regulated by the sympathetic nervous system. The measurement of salivary alpha-amylase activity has been proposed to reflect stress-related changes in the autonomic nervous system, and it may be a good choice for monitoring sympathetic nervous system activity in specialized subjects. Hence, it can be concluded that salivary sAA levels can be taken as a predictable as well as reproducible marker for oral SCC or premalignant lesions.

Keywords: Carcinoma; malignancy; saliva.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
(Group I) clinical picture of the patient in healthy group
Figure 2
Figure 2
(Group II) Clinical picture of the patient in premalignant group
Figure 3
Figure 3
(Group III) Clinical picture of the patient in oral squamous cell carcinoma group
Figure 4
Figure 4
Armamentarium for clinical examination
Figure 5
Figure 5
Armamentarium for sample collection
Figure 6
Figure 6
Collection of saliva sample
Figure 7
Figure 7
Collection of blood sample
Figure 8
Figure 8
(a) Centrifuge machine. (b) Reagent for analysis of salivary alpha-amylase. (c) ELISA plate with wells showing processing of salivary samples. (d) ELISA plate with wells showing processing of plasma samples. (e) ELISA plate reader
Graph 1
Graph 1
Mean age of three groups
Graph 2
Graph 2
Distribution of gender of three groups
Graph 3
Graph 3
Mean plasma α-amylase level of three groups over the periods
Graph 4
Graph 4
For each group, comparison of mean difference in plasma α-amylase level between the periods
Graph 5
Graph 5
For each period, comparison of mean difference in plasma α-amylase level between the groups
Graph 6
Graph 6
Mean saliva α-amylase level of three groups over the periods
Graph 7
Graph 7
For each group, comparison of mean difference in saliva α-amylase level between the periods
Graph 8
Graph 8
For each period, comparison of mean difference in saliva α-amylase levels between the groups
Graph 9
Graph 9
Comparison of α-amylase level of three groups in blood and saliva at morning
Graph 10
Graph 10
Comparison of α-amylase level of three groups in plasma and saliva at afternoon
Graph 11
Graph 11
Comparison of α-amylase level of three groups in plasma and saliva at night
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