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. 2022 Sep 7:5:1445-1451.
doi: 10.1016/j.crfs.2022.08.022. eCollection 2022.

Recovery of value-added anthocyanins from mulberry by a cation exchange chromatography

Zhiwei Liao  1 , Xuan Zhang  1 , Xi Chen  2 , Maurizio Battino  3   4 , Francesca Giampieri  3   4 , Weibin Bai  1 , Lingmin Tian  1
Affiliations

Recovery of value-added anthocyanins from mulberry by a cation exchange chromatography

Zhiwei Liao et al. Curr Res Food Sci. .

Abstract

Anthocyanins are often targets in polyphenol analysis. However, it's hard to effectively separate anthocyanin from copigments such as phenolic acid and flavanols due to their similar structure. Thus, a cation exchange chromatography with 001 × 7 has been developed, which is available for anthocyanins isolation both on a small and large scale. The optimal process condition of anthocyanins isolation was determined. Compared to the macroporous adsorbent resins and Strong Cation Exchange resin (SCX), 001X7 shows greater economic advantages in large-scale purification of anthocyanins. More than 95% purity of the anthocyanin fraction can be achieved through this approach. This method shows a path to provide large quantities of copigments-free anthocyanins from mulberry polyphenols for the further study of its biological effects and may be extended to other analytical methods of polyphenol isolation from other plant materials.

Keywords: 001X7; Anthocyanins; Column chromatography; Copigments; Isolation; Scale-up.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Image 1
Graphical abstract
Fig. 1
Fig. 1
Elution sequence of the purification process of anthocyanins in mulberry extract using 001X7 cation-exchange resin.
Fig. 2
Fig. 2
Identification and quantification of anthocyanins and copigments from black mulberry. HPLC-DAD chromatograms of anthocyanins at 520 nm (A) and copigments at 280 nm (B); LC-MS spectras and structures of cyanidin-3-O-glucoside (C), cyanidin-3-O-rutinoside (D).
Fig. 3
Fig. 3
Distribution of phenolic acid and flavonols in the mulberry extract. n=3, mean ± SD.
Fig. 4
Fig. 4
Effect of different pH of loading eluate solution (A), flow rate during loading (B) and elution steps (C), concentration of NaCl in eluate solution (D) on recovery, adsorption and desorption rate, Static adsorption and desorption (E). n=3, mean ± SD.
Fig. 5
Fig. 5
Retention of copigments (A) and anthocyanins (B) during the column chromatographic separation (large scale). Extract dissolved in methanol/acetic acid 93:7 (v/v) and concentration were at 1.3 g/L.
Fig. 6
Fig. 6
Flowchart for the implementation of adsorptive column chromatographic method for anthocyanins fractionation.
See this image and copyright information in PMC

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