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. 2023;83(1):81-92.
doi: 10.3233/CH-221536.

Transcription factor GATA1 represses oxidized-low density lipoprotein-induced pyroptosis of human coronary artery endothelial cells

Chen Bai  1 , Jiangang Wang  1 , Jingxing Li  1
Affiliations

Transcription factor GATA1 represses oxidized-low density lipoprotein-induced pyroptosis of human coronary artery endothelial cells

Chen Bai et al. Clin Hemorheol Microcirc. 2023.

Retraction in

  • Retraction notice.
    [No authors listed] [No authors listed] Clin Hemorheol Microcirc. 2025 Nov 23:13860291251390410. doi: 10.1177/13860291251390410. Online ahead of print. Clin Hemorheol Microcirc. 2025. PMID: 41275358 No abstract available.

Abstract

Background: Atherosclerosis (AS) is defined as a chronic inflammatory disorder underly the pathogenesis of cardiovascular diseases (CVDs). Endothelial pyroptosis is associated with AS-like diseases and other CVDs.

Objective: This work was designed to expound on the effect of GATA-binding protein 1 (GATA1) on pyroptosis of human coronary artery endothelial cells (HCAECs) in AS.

Methods: HCAECs were treated with oxidized-low density lipoprotein (ox-LDL) to establish HCAEC injury models. Plasmids for overexpressing GATA1 or silencing retinoic acid-related orphan receptor α (RORα) were transfected into HCAECs. Thereafter, the mRNA levels of GATA1 and RORα in HCAECs were detected using real-time quantitative polymerase chain reaction. HCAEC viability was examined using the cell counting kit-8 method. The levels of pyroptosis-related proteins NOD-like receptor protein 3 (NLRP3), cleaved-Caspase-1, N-terminal of gasdermin D (GSDMD-N), and pyroptosis-related inflammatory cytokines interleukin (IL)-1β and IL-18 were determined using Western blot and enzyme-linked immunosorbent assays, respectively. The targeting relationship between GATA1 and RORα was verified using the chromatin-immunoprecipitation assay. Then, the rescue experiment was conducted to explore the effect of RORα on pyroptosis of ox-LDL-treated HCAECs.

Results: In ox-LDL-treated HCAECs, GATA1 and RORα expressions were decreased, HCAEC viability was reduced, and the levels of NLRP3, cleaved-Caspase1, GSDMD-N, IL-1β, and IL-18 were elevated. GATA1 overexpression increased HCAEC viability and attenuated pyroptosis. GATA1 bound to the RORα promoter region to stimulate RORα transcription, and RORα suppression facilitated ox-LDL-induced pyroptosis of HCAECs.

Conclusions: GATA1 activated RORα transcription and therefore limited pyroptosis of ox-LDL-treated HCAECs.

Keywords: GATA1; GSDMD-N; HCAEC; NLRP3; RORα; cleaved-Caspase-1; ox-LDL; pyroptosis.

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