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1 Department of Stem Cell Transplantation (SCT) and Research Department Cell and Gene Therapy, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany
2 Service d’Hematolgie, Hôpital Brabois, CHRU Nancy and Biopole de l’Université de Lorraine, Vandoeuvre les Nancy, France
Book Affiliations
1 Department of Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
2 Bart’s Cancer Institute, Queen Mary University of London, London, UK
3 Institut Paoli-Calmettes Comprehensive Cancer Center Aix-Marseille Université School of Medicine, Marseille, France
4 Maladies du Sang, Unité de Thérapie Cellulaire Centre hospitalier-Universitaire de Lille, Lille, France
5 Department of Internal Medicine II, University Hospital Würzburg, Würzburg, Bayern, Germany
1 Department of Stem Cell Transplantation (SCT) and Research Department Cell and Gene Therapy, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany
2 Service d’Hematolgie, Hôpital Brabois, CHRU Nancy and Biopole de l’Université de Lorraine, Vandoeuvre les Nancy, France
Book Affiliations
1 Department of Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
2 Bart’s Cancer Institute, Queen Mary University of London, London, UK
3 Institut Paoli-Calmettes Comprehensive Cancer Center Aix-Marseille Université School of Medicine, Marseille, France
4 Maladies du Sang, Unité de Thérapie Cellulaire Centre hospitalier-Universitaire de Lille, Lille, France
5 Department of Internal Medicine II, University Hospital Würzburg, Würzburg, Bayern, Germany
CAR-T cell expansion and persistence are critical parameters for therapeutic efficacy and toxicity (Locke et al. 2020). However, CAR-T cells are patient-specific ‘living drugs’ with an unpredictable ability to expand in vivo. Thus, close postinfusion monitoring should be a major prerequisite to better manage this therapy. Critical parameters include CAR-T cell expansion kinetics and phenotype immune reconstitution and serum biomarkers (Fig. 35.1; Kalos et al. 2011; Hu and Huang 2020). Additionally, prospective collection and storage of patient specimens should be planned for future hypothesis-driven studies at specialized research centres. To date, despite the rapid expansion of CAR-T cell therapy, no standard recommendations exist for CAR monitoring, and harmonization of efforts across multiple centres is urgently needed.
Ayuk FA, Berger SC, Badbaran A, et al. Axicabtagene ciloleucel in vivo expansion and treatment outcome in aggressive B-cell lymphoma in a real-world setting. Blood Adv. 2021;5(11):2523–7.
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PMC
-
PubMed
Badbaran A, Berger C, Riecken K, et al. Accurate in-vivo quantification of CD19 CAR-T cells after treatment with axicabtagene ciloleucel (Axi-cel) and tisagenlecleucel (Tisa-cel) using digital PCR. Cancers (Basel). 2020;12:1970.
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PMC
-
PubMed
Campomenosi P, Gini E, Noonan DM, et al. A comparison between quantitative PCR and droplet digital PCR technologies for circulating microRNA quantification in human lung cancer. BMC Biotechnol. 2016;16:60.
-
PMC
-
PubMed
Fehse B, Badbaran A, Berger C, et al. Digital PCR assays for precise quantification of CD19-CAR-T cells after treatment with axicabtagene ciloleucel. Mol Ther Methods Clin Dev. 2020;16:172–8.
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PMC
-
PubMed
