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. 2023 Mar 7;54(2):190-192.
doi: 10.1093/labmed/lmac097.

Comparison of RhD Typing Results by Serology and Molecular Methods

Richard R Gammon  1 , Michelle Conceicao  2 , Nancy Benitez  3 , Frieda Bright  4 , Kelley Counts  5 , Claribel Resto  3 , Karl Rexer  5   6
Affiliations

Comparison of RhD Typing Results by Serology and Molecular Methods

Richard R Gammon et al. Lab Med. .

Abstract

Objective: Molecular testing determines D antigen status when abnormal serologic results are observed. Molecular testing is routinely batched, resulting in longer turnaround time for abnormal D status resolution. During the interim, obstetric patients with questionable/uninterpretable and weak D typing results by serology, per the immunohematology reference laboratory (IRL) policy, will receive RhD negative blood. This study aimed to determine whether serology results achieved a concordance.

Methods: Six hospitals provided samples to the IRL (first IRL) for RhD status by DNA. De-identified samples were sent for serology RhD (second IRL). A concordance of ≥80% was acceptable.

Results: Forty-nine samples were evaluated. Results were concordant (65.3% [32/49]) and discordant (34.7% [17/49]). This is significantly lower than clinically acceptable 80% (z = 2.57, P < .05). The turnaround-time was 3.0 hours for serology and 4.4 days for molecular evaluation.

Conclusion: Due to a low concordance, serology could not be used in place of molecular testing.

Keywords: D-alloimmunization; concordance; immunohematology reference laboratory; molecular; serology; turnaround time.

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