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Review
. 2022 Nov 1:536:104-111.
doi: 10.1016/j.cca.2022.09.014. Epub 2022 Sep 17.

Miniaturized Real-Time PCR systems for SARS-CoV-2 detection at the Point-of-Care

Valeria Garzarelli  1 , Maria Serena Chiriacò  2 , Marco Cereda  3 , Isidora Autuori  4 , Francesco Ferrara  5
Affiliations
Review

Miniaturized Real-Time PCR systems for SARS-CoV-2 detection at the Point-of-Care

Valeria Garzarelli et al. Clin Chim Acta. .

Abstract

Over the past two years, SARS-CoV-2 (Severe Acute Respiratory Syndrome-Coronavirus 2) infection has spread globally causing multi-organ disease and severely impacting the healthcare systems of all countries. Accordingly, the development of easy-to-access diagnostic devices has become essential to limit the effect of the virus worldwide. Real-Time PCR is considered the gold standard to identify SARS-CoV-2 infection due to high sensitivity, affordability, and capacity to detect low viral loads at early disease stage. Advances in lab on a chip technology has led to the development of some Point-of-Care (POC) devices using Real-Time PCR and approved by the United States Food and Drug Administration. We provide an overview on recently developed POC tests for the rapid diagnosis of COVID-19 infection. Practical applications of miniaturized devices based on viral genome amplification as well as favorable features such as reduced sample processing time, ease of use by non-specialized personnel, and the potential of PCR-based POC technologies will be highlighted and reviewed.

Keywords: Lab On Chip; Molecular Biology; Point of Care; Real-Time PCR; SARS-CoV-2; Viral infection.

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Conflict of interest statement

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
Advantages and disadvantages of POC RT-PCR devices .
Fig. 2
Fig. 2
A. POC device and procedure; B. Results of COVID-19 test. Figures reproduced with Copyright permissions from reference .
Fig. 3
Fig. 3
RT-LAMP miniaturized device connected to smartphone via Bluetooth .
See this image and copyright information in PMC

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