Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022:2546:1-12.
doi: 10.1007/978-1-0716-2565-1_1.

Introduction to Mass Spectrometry for Bimolecular Analysis in a Clinical Laboratory

Y Victoria Zhang  1 , Uttam Garg  2
Affiliations

Introduction to Mass Spectrometry for Bimolecular Analysis in a Clinical Laboratory

Y Victoria Zhang et al. Methods Mol Biol. 2022.

Abstract

Mass spectrometry is a technique that identifies analytes based on mass-to-charge (m/z) ratio and structural fragments. Although this technique has been used in research and specialized clinical laboratories for decades, only in recent years has mass spectrometry become popular in routine clinical laboratories. Mass spectrometry, especially when coupled with gas chromatography or liquid chromatography, provides very specific and often sensitive analysis of many analytes. Other advantages of mass spectrometry include simultaneous analysis of multiple analytes (>100) and generally limited requirement for specialized reagents. Commonly measured analytes by mass spectrometry include metabolites, drugs, hormones, and proteins.

Keywords: Clinical laboratory; Endocrinology; Gas chromatography; Hormones and proteins; Liquid chromatography; Mass spectrometry; Newborn screening; Tandem mass spectrometry.

PubMed Disclaimer

References

    1. Strathmann FG, Hoofnagle AN (2011) Current and future applications of mass spectrometry to the clinical laboratory. Am J Clin Pathol 136(4):609–616 - DOI - PubMed
    1. Holmes DT (2019) A brief update on mass spectrometry applications to routine clinical endocrinology. Clin Mass Spectrom 13:18–20 - DOI - PubMed - PMC
    1. Grenga L, Pible O, Armengaud J (2019) Pathogen proteotyping: a rapidly developing application of mass spectrometry to address clinical concerns. Clin Mass Spectrom 14(Pt A):9–17 - DOI - PubMed
    1. Fung AWS, Sugumar V, Ren AH, Kulasingam V (2020) Emerging role of clinical mass spectrometry in pathology. J Clin Pathol 73(2):61–69 - DOI - PubMed
    1. Kebarle P (2000) A brief overview of the present status of the mechanisms involved in electrospray mass spectrometry. J Mass Spectrom 35(7):804–817. https://doi.org/10.1002/1096-9888(200007)35:7<804::Aid-Jms22>3.3.C... - DOI - PubMed

MeSH terms

LinkOut - more resources