Carotane sesquiterpenoids A-G from the desert endophytic fungus Fusarium sp. HM 166

Abstract

Seven undescribed carotane sesquiterpenoids named fusanoids A-G (1-7), along with one known analog (8) and two known sesterterpenes (9 and 10), were isolated from the fermentation broth of the desert endophytic fungi Fusarium sp. HM166. The structures of the compounds, including their absolute configurations, were determined by spectroscopic data, single-crystal X-ray diffraction analysis, and ECD calculations. Compound 10 showed cytotoxic activities against human hepatoma carcinoma cell line (Huh-7) and human breast cell lines (MCF-7 and MDA-MB-231), and compound 2 showed cytotoxic activity against MCF-7, while compounds 4-9 were inactive against all the tested cell lines. Compounds 4 and 10 showed potent inhibitory activities against the IDH1^R132h mutant.

Fig. 1. Structures of compounds 1–10.

Fig. 2. Key ¹H–¹H and HMBC correlations of 1.

Fig. 3. Key ROESY correlations of 1.

Fig. 4. ORTEP drawing of compound 1.

Fig. 5. Calculated and experimental ECD spectra of 1.