Enzyme immunoassay for gentamicin
- PMID: 361288
Enzyme immunoassay for gentamicin
Abstract
We describe a gentamicin assay in which a peroxidase-gentamicin conjugate competes with gentamicin for binding to a gentamicin antibody adsorbed to a polystyrene solid phase. The assay can be completed in 30 min and requires 50 microliter of diluted serum. The precision and accuracy are equivalent to that of a radioimmunoassay technique and the reagents are stable for several months.
Similar articles
-
Homogeneous reactant-labeled fluorescent immunoassay for therapeutic drugs exemplified by gentamicin determination in human serum.Clin Chem. 1977 Aug;23(8):1402-8. Clin Chem. 1977. PMID: 326439
-
Comparison of a fluorescent immunoassay with an enzyme immunoassay and a radioimmunoassay for gentamicin.Am J Hosp Pharm. 1984 Dec;41(12):2647-50. Am J Hosp Pharm. 1984. PMID: 6393760
-
Correlation of bioluminescent assay of gentamicin in serum with agar diffusion assay, latex agglutination inhibition card test, enzyme immunoassay, and fluorescence immunoassay.J Clin Microbiol. 1984 Sep;20(3):396-9. doi: 10.1128/jcm.20.3.396-399.1984. J Clin Microbiol. 1984. PMID: 6436304 Free PMC article.
-
Enzyme immunoassay of gentamicin with use of a centrifugal analyzer.Clin Chem. 1981 Aug;27(8):1460-2. Clin Chem. 1981. PMID: 7023751
-
[Studies for developing hetero-bifunctional reagents and their applications in enzyme immunoassay].Yakugaku Zasshi. 1985 Jun;105(6):517-30. doi: 10.1248/yakushi1947.105.6_517. Yakugaku Zasshi. 1985. PMID: 2933508 Review. Japanese. No abstract available.
Cited by
-
Comparison of radioimmunoassay and enzyme immunoassay methods in determining gentamicin pharmacokinetic parameters and dosages.Antimicrob Agents Chemother. 1982 Oct;22(4):648-51. doi: 10.1128/AAC.22.4.648. Antimicrob Agents Chemother. 1982. PMID: 6758688 Free PMC article.
-
Comparison of different tobramycin assays.J Clin Microbiol. 1982 May;15(5):795-801. doi: 10.1128/jcm.15.5.795-801.1982. J Clin Microbiol. 1982. PMID: 6808011 Free PMC article.
-
Quantitative assay of soluble beta-hemolytic streptococcal antigens via an immunochemical turbidimetric method with a spectrophotometer.J Clin Microbiol. 1979 Sep;10(3):334-8. doi: 10.1128/jcm.10.3.334-338.1979. J Clin Microbiol. 1979. PMID: 385619 Free PMC article.
-
Serum aminoglycoside assay by enzyme-mediated immunoassay (EMIT): correlation with radioimmunoassay, microbiological assays.Antimicrob Agents Chemother. 1981 Jun;19(6):1064-6. doi: 10.1128/AAC.19.6.1064. Antimicrob Agents Chemother. 1981. PMID: 7023380 Free PMC article.
-
Comparison of an HPLC method with a RIA, EMIT and FIA method for the assay of serum gentamicin with extensive statistical evaluation.Pharm Weekbl Sci. 1982 Apr 23;4(2):32-7. doi: 10.1007/BF01963658. Pharm Weekbl Sci. 1982. PMID: 7048242