Complete Genome Sequence of Mycobacteriophage IgnatiusPatJac

Abstract

IgnatiusPatJac is a Siphoviridae mycobacteriophage capable of lytic infection in Mycobacterium smegmatis and Mycobacterium tuberculosis. It was isolated from damp soil in Johannesburg, South Africa. The 51,164-bp double-stranded DNA genome has a GC content of 63.6%, predicted to encode 93 genes. IgnatiusPatJac is classified as an A1 subcluster mycobacteriophage.

FIG 1

Genome organization of mycobacteriophage IgnatiusPatJac. The double-stranded DNA genome of IgnatiusPatJac is represented as a horizontal bar with vertical markers at every kilobase pair. Gene numbers are shown in boxes, which are shaded according to their phamily assignments determined using Phamerator (7). Boxes above and below represent transcription from plus and negative strands, respectively. Putative gene functions, based on BLAST analyses, are indicated. The inset represents a transmission electron micrograph of IgnatiusPatJac (scale bar equivalent to 50 nm). Droplets of purified IgnatiusPatJac lysate were placed onto copper grids (Agar Scientific, UK), coated with carbon, and rendered hydrophilic using an EMS100 Glow Discharge Unit (Electron Microscopy Sciences, USA). Samples were visualized using negative staining with 2% uranyl acetate (SPI Supplies, USA).