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. 2022 Sep 12:2022:6193876.
doi: 10.1155/2022/6193876. eCollection 2022.

Effect of TGF- β 2 on the Mechanical Properties of Posterior Scleral Fibroblasts in Experimental Myopia

Boyu Chen  1   2 , Weyi Chen  3 , Baoyan Han  4 , Chunmei Tong  2 , Jingxue Ma  5
Affiliations

Effect of TGF- β 2 on the Mechanical Properties of Posterior Scleral Fibroblasts in Experimental Myopia

Boyu Chen et al. Biomed Res Int. .

Retraction in

Abstract

Objective: The effects of TGF-β2 on mechanical properties of sclerotic desmocytes isolated from healthy and myopic guinea pigs were investigated in order to further understand the pathogenesis of myopia. To study the effect of TGF-β2 on the mechanical properties of posterior scleral fibroblasts in experimental myopia.

Methods: A lens-induced myopia (LIM) animal model was developed in 12 guinea pigs, with the opposite eye serving as a self-control (SC). Five untreated guinea pigs served as normal controls. Lenses were removed 30 days after model onset. Primary scleral fibroblasts were isolated and passaged twice and then treated with vehicle control or 1, 10, or 100 ng/mL TGF-β2. After 24 h, micropipette aspiration was used to investigate the viscoelastic properties of the cells.

Results: Scleral fibroblasts from LIM exhibited significantly higher equilibrium moduli and apparent viscosities relative to SC without TGF-β2 treatment. Treatment of LIM or SC scleral fibroblasts with 1 or 10 ng/mL TGF-β2 led to significantly different (p < 0.05) equilibrium moduli and apparent viscosities compared with vehicle control, whereas no significant differences were observed upon treatment with 100 ng/mL TGF-β2. LIM cells treated with 1 and 10 ng/mL TGF-β2 exhibited lower equilibrium moduli and apparent viscosities compared with similarly treated SC cells, but LIM cells and SC cells treated with 100 ng/mL TGF-β2 had similar mechanical properties.

Conclusions: The addition of 1 and 10 ng/mL TGF-β2 can lower the equilibrium modulus and apparent viscosity of scleral fibroblasts in the normal eye.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
A micropipette aspiration assay was used to determine mechanical properties. (a) A representative determination of the relationship between the aspiration length and time. (b) The chamber structure.
Figure 2
Figure 2
Morphology of guinea pig scleral fibroblasts. Primary cultures of guinea pig scleral fibroblasts were viewed under a phase contrast microscope at 200x magnification at (a) 3–5 days and (b) 10–14 days after isolation.
Figure 3
Figure 3
Immunocytochemical identification of the primary isolates from guinea pig sclera as desmocytes. Cells cultured for 10 to 14 days were stained for (a) vimentin (+), (b) desmin (−), (c) keratin (−), and (d) S-100 (−) and observed with a light microscope at 400x magnification.
Figure 4
Figure 4
Effects of TGF-β2 on the mean E∞ (a) and μ (b) of the experimental and control scleral fibroblast cells.
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