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. 2022 Sep 13;11(18):2813.
doi: 10.3390/foods11182813.

Hawthorn Juice Simulation System for Pectin and Polyphenol Adsorption Behavior: Kinetic Modeling Properties and Identification of the Interaction Mechanism

Xuan Zhang  1 , Meijiao Li  1 , Wen Zhao  1   2 , Zhe Gao  1 , Mengying Wu  1 , Tong Zhou  1 , Chen Wu  1 , Kaixuan Zhou  1 , Xue Han  1 , Qian Zhou  1   2
Affiliations

Hawthorn Juice Simulation System for Pectin and Polyphenol Adsorption Behavior: Kinetic Modeling Properties and Identification of the Interaction Mechanism

Xuan Zhang et al. Foods. .

Abstract

The interaction between polyphenols and polysaccharides plays an important role in increasing the turbidity stability of fruit juice and improving unpleasant sensory experiences. The binding adsorption behavior between hawthorn pectin (HP) and polyphenols (epicatechin and chlorogenic acid) accorded with the monolayer adsorption behavior driven by chemical action and were better fitted by pseudo-second order dynamic equation and Langmuir model. The HP binding sites (Qm) and adsorption capacity (Qe) to epicatechin were estimated at 75.188 and 293.627 μg/mg HP, respectively, which was about nine and twelve times higher than that of chlorogenic acid. The interaction between HP and polyphenols exhibited higher turbidity characteristics, particle size and lower zeta potential than epicatechin and chlorogenic acid alone. Meanwhile, according to Fourier Transform Infrared Spectroscopy (FT-IR) analysis, it could be speculated that the interaction between HP and polyphenols resulted in chemical combination. Moreover, ΔH < 0 and TΔS < 0, which indicated that the interaction between HP and polyphenols was mainly driven by hydrogen bonds and van der Waals forces.

Keywords: chlorogenic acid; epicatechin; hawthorn; non-covalent interaction; pectin.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
(A) Adsorption kinetics curves of EC and CA; (B) Intragranular diffusion equation fitting; (C) Pseudo-first order dynamic equation fitting; (D) Pseudo-second order dynamic equation. EC: epicatechin; CA: chlorogenic acid.
Figure 2
Figure 2
Effect of solution conditions on adsorption capacity of EC (A) and CA (B). Different letters represent significant differences (p < 0.05). EC: epicatechin; CA: chlorogenic acid.
Figure 3
Figure 3
The average particle size and zeta potential between HP and polyphenols (ratio of 5:1). (A) The zeta potential between HP and polyphenols (EC and CA). (B)The average particle size and polydispersity index (PDI) between HP and EC. (C) The average particle size and polydispersity index (PDI) between HP and CA. HP: hawthorn pectin; EC: epicatechin; CA: chlorogenic acid.
Figure 4
Figure 4
SEM pictures of HP (A), EC (B), CA (C), HP-EC (D), HP-CA (E); and the row exhibited different magnifications are at (1) 200×; (2) 1k×; (3) 5k×. HP: hawthorn pectin EC: epicatechin; CA: chlorogenic acid; HP-EC: the mixture of hawthorn pectin and epicatechin; HP-CA: the mixture of hawthorn pectin and chlorogenic acid.
Figure 5
Figure 5
FT-IR spectrum of HP, EC and HP-EC mixture (A); CA and HP-CA mixture (B). HP: hawthorn pectin EC: epicatechin; CA: chlorogenic acid; HP-EC mixture: the mixture of hawthorn pectin and epicatechin; HP-CA mixture: the mixture of hawthorn pectin and chlorogenic acid.
See this image and copyright information in PMC

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