Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Sep 18;11(18):2896.
doi: 10.3390/foods11182896.

Study on Active Particles in Air Plasma and Their Effect on α-Amylase

Cunshe Chen  1 , Ruohao Sun  1 , Ping Liu  1 , Jufang Yang  1 , Zhixuan Ouyang  1 , Zhihua Pang  1
Affiliations

Study on Active Particles in Air Plasma and Their Effect on α-Amylase

Cunshe Chen et al. Foods. .

Abstract

As a new technology for food processing, plasma has good prospects for protein modification. This study investigated the effect of plasma on the activity of the α-amylase. The composition of the active particles in air plasma generated by spark discharge was analyzed and determined. Furthermore, the quantitative analysis of the active particles such as H2O2, O3, and -OH was made by the chemical detection method. Powdered α-amylase was treated with plasma in various conditions, in which α-amylase and the variation of α-amylase activity under the action of air plasma were quantitatively analyzed. The results showed that the concentration of active particles in the system was positively correlated with the action time for air plasma. After 5 min of plasma action, the concentration of O3 and H2O2 was large enough for food disinfection, but the concentration of -OH was smaller and its lifetime was extremely short. Moreover, it was determined that the optimum action time for the activation of solid powdered α-amylase by air plasma was 120 s. With higher energy, the air plasma acts directly on solid powdered α-amylase to destroy its spatial structure, resulting in enzyme inactivation, sterilization, and disinfection.

Keywords: active particle composition; enzymolysis activity; plasma; α-amylase.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing financial interests or personal relationships that could influence the work reported in this paper.

Figures

Figure 1
Figure 1
The quality of ozone produced by the plasma.
Figure 2
Figure 2
The quality of hydrogen peroxide produced by the plasma.
Figure 3
Figure 3
The quality of hydroxyl radical produced by the plasma.
Figure 4
Figure 4
The relationship between α-amylase enzyme activity and duration of action.
Figure 5
Figure 5
The diagram of α-amylase enzyme activity affected by plasma after 24 h and 1 week.
Figure 6
Figure 6
The relationship between α-amylase enzyme activity and duration of action.
Figure 7
Figure 7
The diagram of low-pressure low-temperature α-amylase enzyme activity affected by plasma after 1 week.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Liu T., Zeng Y., Xue X., Sui Y., Liang Y., Wang F., Feng F., Kim J. He-Plasma Jet Generation and Its Application for E. coli Sterilization. J. Spectrosc. 2021;2021:1–10. doi: 10.1155/2021/6671531. - DOI
    1. Moreau M., Orange N., Feuilloley M.G. Non-thermal plasma technologies: New tools for bio-decontamination. Biotechnol. Adv. 2008;26:610–617. doi: 10.1016/j.biotechadv.2008.08.001. - DOI - PubMed
    1. Misra N.N., Tiwari B.K., Raghavarao K.S.M.S., Cullen P.J. Nonthermal Plasma Inactivation of Food-Borne Pathogens. Food Eng. Rev. 2011;3:159–170. doi: 10.1007/s12393-011-9041-9. - DOI
    1. Mackinder M.A., Wang K., Zheng B., Shrestha M., Fan Q.H. Magnetic field enhanced cold plasma sterilization. Plasma Med. 2020;17–18:100092. doi: 10.1016/j.cpme.2019.100092. - DOI
    1. Lee Y., Lee Y.Y., Kim Y.S., Balaraju K., Mok Y.S., Yoo S.J., Jeon Y. Enhancement of seed germination and microbial disinfection on ginseng by cold plasma treatment. J. Ginseng. Res. 2021;45:519–526. doi: 10.1016/j.jgr.2020.12.002. - DOI - PMC - PubMed