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. 2022 Sep 19;11(18):2924.
doi: 10.3390/foods11182924.

Prevalence of Multidrug-Resistant Salmonella enterica Serovars in Buffalo Meat in Egypt

Samir Mohammed Abd-Elghany  1 , Takwa Mohammed Fathy  1 , Amira Ibrahim Zakaria  1 , Kálmán Imre  2 , Adriana Morar  2 , Viorel Herman  3 , Raul Pașcalău  4 , Laura Șmuleac  5 , Doru Morar  6 , Mirela Imre  7 , Khalid Ibrahim Sallam  1
Affiliations

Prevalence of Multidrug-Resistant Salmonella enterica Serovars in Buffalo Meat in Egypt

Samir Mohammed Abd-Elghany et al. Foods. .

Abstract

The current study aimed to investigate the presence of Salmonella spp. prevalence in buffalo meat in Egypt, along with studying the antimicrobial susceptibility of the recovered isolates. Salmonella spp. was detected in 25% of tested buffalo meat. A total of 53 (100%) isolates were genetically verified by PCR as Salmonella, based on the detection of the invA gene. The stn and hilA genes were detected in 71.7% (38/53), and 83.0% (44/53) of the recovered isolates, respectively. Salmonella Enteritidis (11/53; 20.7%) was the most commonly isolated serovar, followed by S. Typhimurium (9/53; 17%), S. Montevideo (6/53; 11.3%), meanwhile, S. Chester, S. Derby, S. Papuana, and S. Saintpaul were the least commonly identified serovars (a single strain for each; 1.9%). Among the 16 antimicrobials tested, amikacin, imipenem, gentamicin, cefotaxime, meropenem, ciprofloxacin, and enrofloxacin were the most effective drugs, with bacterial susceptibility percentages of 98.1%, 94.3%, 92.5%, 86.8%, 83.0%, 73.6%, and 69.8%, respectively. Meanwhile, the least effective ones were erythromycin, streptomycin, clindamycin, cefepime, and nalidixic acid, with bacterial resistance percentages of 100%, 98.1%, 88.7%, 77.4%, and 66%, respectively. Interestingly, the high contamination level of Egyptian buffalo meat with multidrug-resistant Salmonella (79.2%; 42/53) can constitute a problem for public health. Therefore, programs to control Salmonella contamination are needed in Egypt.

Keywords: PCR; Salmonella serovars; antimicrobial resistance; buffalo meat; virulence genes.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Distribution of the identified Salmonella serovars (n = 53) isolated from buffalo meat samples.
Figure 2
Figure 2
Agarose gel electrophoresis of multiplex PCR amplicons of invA (275 bp), stn (617 bp), and hilA (854 bp) virulence genes of Salmonella spp. Lane M: 100-bp ladder as a molecular size DNA marker (Jena Bioscience GmbH). Lane C+: Control positive strain for invA, stn, and hilA genes. Lane C–: Control negative. Lanes 1 (S. Enteritidis), 2 (S. Typhimurium), 5 (S. Infantis), 9 (S. Dublin), 10 (S. Anatum), 11 (S. Derby), and 12 (S. Papuana) showed positive bands for invA, stn and hilA genes. Lanes 3 (S. Montevideo), 7 (S. Essen), and 14 (S. Chester) showed positive bands for invA and stn genes. Lanes 4 (S. Rissen), 6 (S. Virchow), 8 (S. Tsevie), and 13 (S. Saintpaul) showed positive bands for invA and hilA genes.
Figure 3
Figure 3
Prevalence of virulence genes among the screened Salmonella isolates (n = 53).
See this image and copyright information in PMC

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