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. 2022 Sep 7;19(18):11245.
doi: 10.3390/ijerph191811245.

Quantifying the Relationship between SARS-CoV-2 Wastewater Concentrations and Building-Level COVID-19 Prevalence at an Isolation Residence: A Passive Sampling Approach

Patrick T Acer  1 , Lauren M Kelly  2 , Andrew A Lover  1 , Caitlyn S Butler  2
Affiliations

Quantifying the Relationship between SARS-CoV-2 Wastewater Concentrations and Building-Level COVID-19 Prevalence at an Isolation Residence: A Passive Sampling Approach

Patrick T Acer et al. Int J Environ Res Public Health. .

Abstract

SARS-CoV-2 RNA loads can be detected in the excreta of individuals with COVID-19 and have demonstrated positive correlations with clinical infection trends. Consequently, wastewater-based epidemiology (WBE) approaches have been implemented globally as a public health surveillance tool to monitor community-level prevalence of infections. The majority of wastewater specimens are gathered as either composite samples via automatic samplers (autosamplers) or grab samples. However, autosamplers are expensive and can be challenging to maintain in cold weather, while grab samples are particularly susceptible to temporal variation when sampling sewage directly from complex matrices outside residential buildings. Passive sampling can provide an affordable, practical, and scalable sampling system while maintaining a reproducible SARS-CoV-2 signal. In this regard, we deployed tampons as passive samplers outside of a COVID-19 isolation unit (a segregated residence hall) at a university campus from 1 February 2021-21 May 2021. Samples (n = 64) were collected 3-5 times weekly and remained within the sewer for a median duration of 24 h. SARS-CoV-2 RNA was quantified using reverse-transcription quantitative polymerase chain reaction (RT-qPCR) targeting the N1 and N2 gene fragments. We quantified the mean viral load captured per individual and the association between the daily viral load and total persons, adjusting for covariates using multivariable models to provide a baseline estimate of viral shedding. Samples were processed through two distinct laboratory pipelines on campus, yielding highly correlated N2 concentrations. Data obtained here highlight the success of passive sampling utilizing tampons to capture SARS-CoV-2 in wastewater coming from a COVID-19 isolation residence, indicating that this method can help inform building-level public health responses.

Keywords: COVID-19; SARS-CoV-2; college campus monitoring; fecal shedding; passive sampling; wastewater-based epidemiology.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
N1 and N2 daily viral loads from the COVID-19 isolation residence, 1 February 2021–21 May 2021 (n = 64).
Figure 2
Figure 2
Correlation between independent average N2 log-transformed viral concentrations on identical samples utilizing separate processing and analysis pipelines (n = 64).
Figure 3
Figure 3
Violin plot showing distributions of N1 and N2 average daily wastewater viral loads per individual in isolation from 1 February 2021–21 May 2021. Note: Markers shown are median, 25th and 75th quantiles.
Figure 4
Figure 4
Violin plot showing distribution of average daily building-level water use per individual in isolation from 1 February 2021–21 May 2021. Note: Markers shown are median, 25th and 75th quantiles.
Figure 5
Figure 5
Total daily isolation building occupancy (line) plotted with N1 and N2 gene copies/day (bars) during the Spring 2021 academic semester. Note: Both SARS-CoV-2 daily wastewater viral loads and daily isolation residence occupancy totals are reported on linear y-axes (n = 64).
Figure 6
Figure 6
Observed COVID-19 isolation residence occupancy plotted against predicted COVID-19 isolation residence occupancy using negative binomial modeling with Spring 2021 data.
See this image and copyright information in PMC

Update of

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