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. 1987 Jan;24(1):91-6.
doi: 10.1016/0161-5890(87)90115-5.

A monoclonal antibody which can distinguish between the two isotypes of human C4

L M Hsiung, D W Mason, A W Dodds

A monoclonal antibody which can distinguish between the two isotypes of human C4

L M Hsiung et al. Mol Immunol. 1987 Jan.

Abstract

A monoclonal antibody to Human C4 (L003) has been shown to bind to the polymorphic C4d fragment of the alpha-chain of C4. Another monoclonal antibody (L001) was shown to bind to the beta-chain. L003 reacts differently with the two isotypes of C4, C4-A and C4-B. The equilibrium constant of L003 for C4-B is approx. 7-fold higher than that for C4-A, while L001 has similar affinity for both C4 types. Also, L003 binding to C4-A is very much more pH dependent than is its binding to C4-B. These observations explain the very useful property of L003 in being able to separate the two isotypes by affinity chromatography.

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