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. 2022 Sep 8;27(18):5809.
doi: 10.3390/molecules27185809.

An Investigation of the Anti-Depressive Properties of Phenylpropanoids and Flavonoids in Hemerocallis citrina Baroni

Tiancheng Ma  1   2 , Yu Sun  2 , Lida Wang  1 , Jinyu Wang  3 , Bo Wu  3 , Tingxu Yan  3 , Ying Jia  3
Affiliations

An Investigation of the Anti-Depressive Properties of Phenylpropanoids and Flavonoids in Hemerocallis citrina Baroni

Tiancheng Ma et al. Molecules. .

Abstract

The World Health Organization predicts that over the next several years, depression will become the most important mental health issue globally. Growing evidence shows that the flower buds of Hemerocallis citrina Baroni (H. citrina) possess antidepressant properties. In the search for new anti-depression drugs, a total of 15 phenylpropanoids and 22 flavonoids were isolated and identified based on spectral data (1D and 2D NMR, HR-ESI-MS, UV) from H. citrina. Among them, compound 8 was a novel compound, while compounds 1-4, 6, 9, 10, 15, 17, 24-26, 28, and 37 were isolated for the first time from Hemerocallis genus. To study the antidepressant activity of phenylpropanoids and flavonoids fractions from H. citrina, macroporous resin was used to enrich them under the guidance of UV characteristics. UHPLC-MS/MS was applied to identify the constituents of the enriched fractions. According to behavioral tests and biochemical analyses, it showed that phenylpropanoid and flavonoid fractions from H. citrina can improve the depressive-like mental state of chronic unpredictable mild stress (CUMS) rats. This might be accomplished by controlling the amounts of the inflammatory proteins IL-6, IL-1β, and TNF-α in the hippocampus as well as corticosterone in the serum. Thus, the monomer compounds were tested for their anti-neuroinflammatory activity and their structure-activity relationship was discussed in further detail.

Keywords: Hemerocallis citrina Baroni; anti-neuroinflammatory activity; antidepressant activity; flavonoids; phenylpropanoids.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Key HMBC correlations of compound 8.
Figure 2
Figure 2
Structures of the isolated compounds.
Figure 3
Figure 3
TIC spectra of HFPE (A) and HFFE (B).
Figure 4
Figure 4
Trends in weight growth rate in each group before and after CUMS treated. The values were represented as mean ± SD (n = 10). ** p < 0.01 compared with the control group, ## p < 0.01 compared with the model group.
Figure 5
Figure 5
Effects of HFPE and HFFE on the sucrose preference before and after CUMS procedure. The values were represented as mean ± SD (n = 10). ** p < 0.01 compared with the control group, ## p < 0.01 compared with the model group.
Figure 6
Figure 6
Effects of HFPE and HFFE on OFT test. (A) represents crossing score, (B) represents rearing score. Results were represented as mean ± SD (n = 10). ** p < 0.01 compared with the control group, # p < 0.05 and ## p < 0.01 compared with the model group.
Figure 7
Figure 7
Effects of HFPE and HFFE on FST. Results were represented as mean ± SD (n = 10). * p < 0.05 compared with the control group, # p < 0.05 and ## p < 0.01 compared with the model group.
Figure 8
Figure 8
Effects of HFPE and HFFE on serum CORT level and the inflammatory level in hippocampus of CUMS rats. ELISA for detecting CORT (A), IL-6 (B), IL-1β (C), and TNF-α (D) levels. Results were represented as mean ± SD (n = 8). ** p < 0.01 compared with the control group, # p < 0.05 and ## p < 0.01 compared with the model group.
Figure 9
Figure 9
Schedule of the experimental procedure.
See this image and copyright information in PMC

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