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. 2022 Sep 8;27(18):5837.
doi: 10.3390/molecules27185837.

Identification of Coumarins and Antimicrobial Potential of Ethanolic Extracts of Dipteryx odorata and Dipteryx punctata

Bruna Cristine Martins de Sousa  1   2 , Santana Pinto de Castro  3 , Katiane Araújo Lourido  1 , Aline Aparecida München Kasper  2 , Geomarcos da Silva Paulino  2 , Camila Delarmelina  4 , Marta Cristina Teixeira Duarte  4 , Adilson Sartoratto  4 , Thiago Almeida Vieira  1   2 , Denise Castro Lustosa  1 , Lauro Euclides Soares Barata  2
Affiliations

Identification of Coumarins and Antimicrobial Potential of Ethanolic Extracts of Dipteryx odorata and Dipteryx punctata

Bruna Cristine Martins de Sousa et al. Molecules. .

Abstract

Dipteryx odorata and Dipteryx punctata are species native to the Amazonian, traded by extractivists to obtain coumarin. We aimed to analyze the presence of coumarin in the ethanolic extracts of leaves, branches and fruits of D. odorata and D. punctata and to evaluate the antimicrobial activity of these extracts against phytopathogenic fungi and bacteria of clinical interest. Chemical analyses were performed by thin layer chromatography (TLC) and by gas chromatography coupled to mass spectrometry (GC-MS). For the antifungal assays, the fungi used were Cercospora longissima, Colletotrichum gloeosporioides, two isolates of Fusarium spp. and Sclerotium rolfsii, and the antibacterial assay was performed using the minimum inhibitory concentration (MIC) test with Burkholderia cepacia, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus bacteria. In D. odorata seed extracts and in D. punctata husks, endocarps, and seeds, we identified 1,2-benzopyrone. D. odorata endocarp extracts and D. punctata seeds provided the greatest decrease in mycelial growth of the evaluated phytopathogens, showing promise as an alternative control. The husk and endocarp extracts of both species had a weak effect on E. coli. This research is the first to compare the different parts of species of the genus Dipteryx and to evaluate the use of husks and endocarps of D. punctata fruits to obtain coumarin. Chemical analyses used to quantify the compounds existing in the extracts, and tests with phytopathogens in vitro and in vivo are currently being carried out.

Keywords: antimicrobial activity; chromatography; cumaru extracts; natural products.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure A1
Figure A1
Dipteryx odorata leaves extract chromatogram.
Figure A2
Figure A2
Dipteryx punctata leaves extract chromatogram.
Figure A3
Figure A3
Dipteryx odorata branches extract chromatogram.
Figure A4
Figure A4
Dipteryx punctata branches extract chromatogram.
Figure A5
Figure A5
Dipteryx odorata husks extract chromatogram.
Figure A6
Figure A6
Dipteryx punctata husks extract chromatogram.
Figure A7
Figure A7
Dipteryx odorata endocarps extract chromatogram.
Figure A8
Figure A8
Dipteryx punctata endocarps extract chromatogram.
Figure A9
Figure A9
Dipteryx odorata seeds extract chromatogram.
Figure A10
Figure A10
Dipteryx punctata seeds extract chromatogram.
See this image and copyright information in PMC

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