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. 2022 Sep 11;11(18):2371.
doi: 10.3390/plants11182371.

Phytochemical and Pharmacological Research in Agrimonia eupatoria L. Herb Extract with Anti-Inflammatory and Hepatoprotective Properties

Natalia Huzio  1 , Andriy Grytsyk  1 , Ain Raal  2 , Lyubov Grytsyk  1 , Oleh Koshovyi  3
Affiliations

Phytochemical and Pharmacological Research in Agrimonia eupatoria L. Herb Extract with Anti-Inflammatory and Hepatoprotective Properties

Natalia Huzio et al. Plants (Basel). .

Abstract

The most promising plant from the genus Agrimony (Agrimonia L.) of the Rosaceae family for use in medical practice is Agrimonia eupatoria L. Phytochemical and pharmacological research in Agrimonia eupatoria L. herb extract, obtained with using 40% ethanol solution as an extractant were carried out. A total of 11 free and 17 bound monosaccharides, 17 amino acids were found in the studied extract, 9 of which are essential. Gallic and ellagic acids, gallocatechin, epigallocatechin, catechin, epicatechin, and epicatechin gallate were identified in the extract of A. eupatoria by the HPLC method; as well as hydroxycinnamic acids: hydroxyphenylacetate, caffeic, syringic, p-coumaric, ferulic, sinapic, cinnamic and quinic acid; flavonoids: quercetin-3-D-glucoside (isoquercitrin), neohesperidin, naringenin, luteolin were found, and their quantitative content was determined, as well by spectrophotometric methods. The herb extract of A. eupatoria belongs to practically non-toxic substances and has pronounced anti-inflammatory (at a dose of 10.0 mg/kg anti-exudative activity reached a maximum in 5 h (88.17%)) and hepatoprotective activity (at a dose of 25 mg/kg it reduce AlAt level by 1.1 and 1.2 times, respectively; AsAt by 1.2 and 1.1 times, respectively), reduces the level of lipid peroxidation and stabilizes the membrane structures of liver cells. Thus, the herb extract of A. eupatoria is a promising substance for the creation of phytomedicines with anti-inflammatory and hepatoprotective activity.

Keywords: Agrimonia eupatoria L.; biological active substances; extract; hepatoprotective activity; herb.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
HPLC-chromatogram of hydroxycinnamic acids of the herb extract of A. eupatoria.
Figure 2
Figure 2
HPLC-chromatogram of the flavonoids of the herb extract of A. eupatoria.
Figure 3
Figure 3
The effect of the herb extract of A. eupatoria on the development of limb swelling in rats.
See this image and copyright information in PMC

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