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. 2023:2576:225-232.
doi: 10.1007/978-1-0716-2728-0_18.

Radiometric Assay of NAPE-PLD Activity

Filomena Fezza  1 , Nicolina Mastrangelo  2 , Mauro Maccarrone  3   4
Affiliations

Radiometric Assay of NAPE-PLD Activity

Filomena Fezza et al. Methods Mol Biol. 2023.

Abstract

N-Acyl-phosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a prominent enzyme involved in the biosynthesis of fatty acid amides, a family of bioactive lipids including anandamide as the prototypical member. Here, we describe a NAPE-PLD assay based on radioactive substrates and product separation by thin layer chromatography (TLC).

Keywords: Anandamide; Fatty acid amides; N-Acyl-phosphatidylethanolamines-hydrolyzing phospholipase D; N-Acylphosphatidylethanolamines; N-Palmitoylethanolamine; Radioactive assay; Thin layer chromatography.

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References

    1. Okamoto Y, Morishita J, Tsuboi K et al (2004) Molecular characterization of a phospholipase D generating anandamide and its congeners. J Biol Chem 279:5298–5305 - DOI
    1. Balvers MG, Verhoeckx KC, Meijerink J et al (2013) Measurement of palmitoylethanolamide and other N-acylethanolamines during physiological andpathological conditions. CNS Neurol Disord Drug Targets 12:23–33 - DOI
    1. Fezza F, Gasperi V, Mazzei C et al (2005) Radiochromatographic assay of N-acyl-phosphatidylethanolamine-specific phospholipase D activity. Anal Biochem 339:113–120 - DOI
    1. Bisogno T, Maccarrone M (2013) Latest advances in the discovery of fatty acid amide hydrolase inhibitors. Expert Opin Drug Discov 8:509–522 - DOI
    1. Tsuboi K, Okamoto Y, Ikematsu N et al (2011) Enzymatic formation of N-acylethanolamines from N-acylethanolamine plasmalogen through N-acylphosphatidylethanolamine-hydrolyzing phospholipase D-dependent and -independent pathways. Biochim Biophys Acta 1811:565–577 - DOI

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