Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023:2566:133-139.
doi: 10.1007/978-1-0716-2675-7_10.

Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin-Embedded Tissue

Sabina Berezowska  1 , José A Galván  2
Affiliations

Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin-Embedded Tissue

Sabina Berezowska et al. Methods Mol Biol. 2023.

Abstract

Autophagy is a highly conserved cellular mechanism of "self-digestion," ensuring cellular homeostasis and playing a role in many diseases including cancer. As a stress response mechanism, it may also be involved in cellular response to therapy. LC3 and Sequestosome 1 (p62/SQSTM1) are among the most widely used markers to monitor autophagy and can be visualized in formalin-fixed and paraffin-embedded tissue by immunohistochemistry. Here we describe a validated staining protocol using an automated staining system available in many routine pathology laboratories, enabling high-throughput staining under standardized conditions.

Keywords: Autophagy; Immunohistochemistry; LC3; Sequestosome 1; p62/SQSTM1.

PubMed Disclaimer

References

    1. Mizushima N, Levine B, Cuervo AM, Klionsky DJ (2008) Autophagy fights disease through cellular self-digestion. Nature 451(7182):1069–1075 - DOI
    1. Scarlatti F, Granata R, Meijer AJ, Codogno P (2009) Does autophagy have a license to kill mammalian cells? Cell Death Differ 16(1):12–20 - DOI
    1. Tschan MP, Simon HU (2010) The role of autophagy in anticancer therapy: promises and uncertainties. J Intern Med 268(5):410–418 - DOI
    1. Feng Y, He D, Yao Z, Klionsky D (2014) The machinery of macroautophagy. Cell Res 24(1):24–41 - DOI
    1. Hurley JH, Schulman BA (2014) Atomistic autophagy: the structures of cellular self-digestion. Cell 157(2):300–311 - DOI

Publication types

LinkOut - more resources