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. 2023:2566:269-279.
doi: 10.1007/978-1-0716-2675-7_22.

Live Fluorescence Visualization of Cellulose and Pectin in Plant Cell Walls

Youssef Chebli  1 , Anja Geitmann  2
Affiliations

Live Fluorescence Visualization of Cellulose and Pectin in Plant Cell Walls

Youssef Chebli et al. Methods Mol Biol. 2023.

Abstract

The plant cell wall comprises various types of macromolecules whose abundance and spatial distribution change dynamically and are crucial for plant architecture. High-resolution live cell imaging of plant cell wall components is, therefore, a powerful tool for plant cell biology and plant developmental biology. To acquire suitable data, the experimental setup for staining and imaging of non-fixed samples must be straightforward and avoid creating stress-induced artifacts. We present a detailed sample preparation and live image acquisition protocol for fluorescence visualization of cell wall components using commercially available probes and stains.

Keywords: Arabidopsis; Cell wall; Cellulose; Confocal laser scanning microscope; Fluorescence; Live cell imaging; Onion; Pectin; Plant development; Polysaccharides; Staining.

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