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Review
. 2022 Sep;40(9):773-781.
doi: 10.3724/SP.J.1123.2022.08001.

[Recent advances in clustered regularly interspaced short palindromic repeats-based detection of severe acute respiratory syndrome coronavirus 2]

[Article in Chinese]
Wen Zhou  1   2 , Kaiguang Yang  1 , Lihua Zhang  1 , Zhen Liang  1 , Yukui Zhang  1
Affiliations
Review

[Recent advances in clustered regularly interspaced short palindromic repeats-based detection of severe acute respiratory syndrome coronavirus 2]

[Article in Chinese]
Wen Zhou et al. Se Pu. 2022 Sep.

Abstract
in English, Chinese

The rapid global spread of coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has introduced various challenges in global public health systems. The poor applicability and sensitivity of the reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and antigen-based tests, as well as the persistent emergence of SARS-CoV-2 variants with different mutations hinder satisfactory epidemic prevention and control. Therefore, there is an urgent need for diagnostic technologies capable of distinguishing SARS-CoV-2 variants with high sensitivity and low (or no) equipment dependence. Diagnosis based on clustered regularly interspaced short palindromic repeats (CRISPR) has low equipment requirements and is programmable, sensitive, and easy to use. Various nucleic acid detection tools with great clinical potential have been developed for the diagnosis of infectious diseases. Therefore, this review focuses on the reported state-of-the-art CRISPR diagnostic technologies developed for the detection and differentiation of SARS-CoV-2 variants, summarizes their characteristics and provides an outlook for their development.

严重急性呼吸综合征冠状病毒2(SARS-CoV-2)导致的新冠肺炎(COVID-19)迅速蔓延全球,给全球公共卫生系统带来了挑战。由于逆转录-定量聚合酶链反应(RT-qPCR)和抗原测试的普遍适用性和灵敏度较差,并且具有不同突变的SARS-CoV-2变体持续的出现,给疫情防控带来了更大的挑战,因此,高灵敏度、无需设备并且能够区分SARS-CoV-2变体的诊断方法亟须发展。基于成簇的规则间隔短回文重复序列(CRISPR)的诊断对设备要求低,具有可编程性、灵敏性和易用性,已经发展出多种核酸检测工具用于传染病的诊断,其在临床上具有巨大的应用潜力。文章聚焦于近期发表的基于CRISPR实现SARS-CoV-2检测和变体区分的最新技术,总结其特点并对其发展进行了展望。

Keywords: SARS-CoV-2 variants; clustered regularly interspaced short palindromic repeats (CRISPR); isothermal nucleic acid amplification; severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

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Figures

Fig. 1
图 1. CARMEN中芯片功能详细原理图[13]
Fig. 2
图 2. CARMEN v.1和mCARMEN工作流程示意图[17]
Fig. 3
图 3. 简化的mCARMEN工作流程示意图[17]
Fig. 4
图 4. SHINE示意图[9]
Fig. 5
图 5. SHINE v.2工作流程示意图[23]
Fig. 6
图 6. ADESSO在临床样本中检测SARS-CoV-2的实验工作流程图[26]
See this image and copyright information in PMC

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References

    1. Larremore D B, Wilder B, Lester E, et al.. Sci Adv, 2021, 7(1): eabd5393 - PMC - PubMed
    1. Mogling R, Meijer A, Berginc N, et al.. Emerg Infect Dis, 2020, 26(8): 1944 - PMC - PubMed
    1. Mina M J, Andersen K G. Science, 2021, 371(6525): 126 - PubMed
    1. Pray I W, Ford L, Cole D, et al.. MMWR Morb Mortal Wkly Rep, 2021, 69(5152): 1642 - PMC - PubMed
    1. Dao Thi V L, Herbst K, Boerner K, et al.. Sci Transl Med, 2020, 12(556): eabc7075 - PMC - PubMed

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